| Control of meningioma cell growth by platelet-derived growth factor (PDGF). | |
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MedLine Citation:
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PMID: 7595638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined the possible involvement of PDGF and PDGF receptors in the growth control of five meningiomas, analyzing the biopsy specimens and the primary cultures derived from the same tumors. Light and electron microscopy demonstrated that MAbs against PDGF beta-receptors immunodecorate meningioma cells in vivo and in vitro, while those against alpha-receptors gave negative results. The effects of PDGF isoforms AA, AB, BB and of PDGF neutralizing antibodies on meningioma cultures were examined using [3H]thymidine incorporation analysis. Only with PDGF-AB and -BB a mitogenic effect was observed, while PDGF-neutralizing antibodies produced a reduction of [3H]thymidine incorporation. The production of PDGF-like growth factors by meningioma cells was tested analyzing the effects of meningioma culture-conditioned media on the growth of Swiss 3T3 cells. In all cases meningioma conditioned media stimulated the in vitro growth of 3T3 fibroblasts and this stimulatory effect was strongly reduced by PDGF-neutralizing antibodies. Furthermore, Northern blot analysis demonstrated expression of c-sis/PDGF-B and PDGF beta-receptors mRNA in all meningioma biopsies and in all the derived cultures. Our results provide strong evidence that PDGF-B chain and PDGF beta-receptors are involved in growth control mechanisms of human meningiomas through autocrine and/or paracrine mechanisms. |
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Authors:
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A Mauro; A Di Sapio; C Mocellini; D Schiffer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the neurological sciences Volume: 131 ISSN: 0022-510X ISO Abbreviation: J. Neurol. Sci. Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1995-11-28 Completed Date: 1995-11-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375403 Medline TA: J Neurol Sci Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 135-43 Citation Subset: IM |
Affiliation:
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2nd Department of Neurology, University of Turin, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Blotting, Northern Cell Division / drug effects, physiology Culture Media, Conditioned Gene Expression / physiology Humans Meningioma / pathology* Microscopy, Immunoelectron Neutralization Tests Platelet-Derived Growth Factor / immunology, pharmacology* Receptor, Platelet-Derived Growth Factor beta Receptors, Platelet-Derived Growth Factor / genetics, immunology, physiology Thymidine Tritium Tumor Cells, Cultured / cytology, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Culture Media, Conditioned; 0/Platelet-Derived Growth Factor; 10028-17-8/Tritium; 50-89-5/Thymidine; EC 2.7.10.1/Receptor, Platelet-Derived Growth Factor beta; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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