| Control of magnesium-protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Role of light, oxygen, and electron and energy transfer. | |
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MedLine Citation:
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PMID: 4202754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Magnesium-protoporphyrin chelatase activity, previously shown in whole cells of Rhodopseudomonas spheroides, could not be demonstrated in cell-free extracts prepared in different ways, although spheroplasts retained moderate activity. Slight activity was detected also in whole cells of Rhodospirillum rubrum. 2. The effects on the activity of the enzyme of inhibitors of electron and energy transfer were studied in whole cells of Rps. spheroides. Amytal, rotenone, azide and cyanide inhibited at low pO(2) in the dark but not under anaerobic conditions in the light. Antimycin A and 2-heptyl-4-hydroxyquinoline N-oxide, as well as uncouplers and oligomycin, inhibited under all environmental conditions. 3. The effects on magnesium chelatase activity of intermediates of the tricarboxylic acid cycle, of thenoyltrifluoroacetone, of a number of artificial electron donors or acceptors, of various quinones and of the oxidation-reduction indicator dyes Benzyl Viologen and Methyl Viologen are described. 4. It was concluded that electron transport between a b-type and a c-type cytochrome as well as associated energy conservation and transformation reactions were essential for activity. There was also a specific requirement for ATP. 5. Exogenous protoporphyrin and magnesium protoporphyrin monomethyl ester were incorporated into bacteriochlorophyll or late precursors by whole cells. 6. Evidence is presented that the insertion of magnesium was the only step inhibited by oxygen in the biosynthetic pathway between protoporphyrin and bacteriochlorophyll. |
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Authors:
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A Gorchein |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 134 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1973 Aug |
Date Detail:
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Created Date: 1974-02-12 Completed Date: 1974-02-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 833-45 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amobarbital
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pharmacology Antimycin A / pharmacology Azides / pharmacology Carbon Radioisotopes Citric Acid Cycle Cyanides / pharmacology Cytochromes / metabolism Electron Transport Energy Transfer Light* Magnesium / metabolism* Methionine / metabolism Oligomycins / pharmacology Oxidation-Reduction Oxygen / metabolism, pharmacology* Rhodobacter sphaeroides / enzymology* Rhodospirillum rubrum / enzymology Rotenone / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Azides; 0/Carbon Radioisotopes; 0/Cyanides; 0/Cytochromes; 0/Oligomycins; 57-43-2/Amobarbital; 63-68-3/Methionine; 642-15-9/Antimycin A; 7439-95-4/Magnesium; 7782-44-7/Oxygen; 83-79-4/Rotenone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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