Document Detail

Control of intracellular movement of connexins by E-cadherin in murine skin papilloma cells.
MedLine Citation:
PMID:  11640887     Owner:  NLM     Status:  MEDLINE    
The gap junctional intercellular communication-deficient mouse skin papilloma cell line P3/22 expresses Cx43 but not E-cadherin. The E-cadherin gene-transfected cells (P3E1) communicate in a calcium-dependent manner and they were used to study how E-cadherin restores the function of connexins. At low calcium, Cx43 molecules remain in the cytoplasm of P3E1 cells and appear at cell-cell contact areas only in high-calcium medium. While Cx43 is unphosphorylated in P3E1 cells in low-calcium medium, two phosphorylated bands appeared at high calcium. However, when Cx26, which has no C-terminal tail that can undergo phosphorylation, was expressed in P3E1 cells, this connexin also moved to the plasma membrane after the calcium shift and partly colocalized with Cx43, suggesting that C-terminal phosphorylation is not essential for E-cadherin-mediated intracellular transport of connexins. In low calcium, both Cx26 and Cx43 remained and colocalized in the endoplasmic reticulum. As early as 30 min after the shift to high-calcium medium, both Cx43 and Cx26 began to accumulate in the Golgi apparatus. Intracellular movement of connexins to the cytoplasmic membrane at high calcium was effectively blocked by cytochalasin D and brefeldin A. These results suggest that E-cadherin junction formation at high calcium leads to formation of actin cables, which directly or indirectly transport connexins from the cytoplasm to the cell-cell contact membranes via the Golgi apparatus.
F J Hernandez-Blazquez; P P Joazeiro; Y Omori; H Yamasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  270     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-19     Completed Date:  2001-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-47     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Unit of Multistage Carcinogenesis, International Agency for Research on Cancer, 150 cours Albert Thomas, Lyon Cedex 08, 69372, France.
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MeSH Terms
Actins / metabolism
Adherens Junctions / chemistry,  metabolism,  ultrastructure
Cadherins / analysis,  genetics,  metabolism*
Calcium / pharmacology
Cell Adhesion / physiology
Cell Communication / physiology
Cell Membrane / metabolism,  ultrastructure
Connexin 43 / analysis,  metabolism*
Connexins / metabolism
Culture Media / pharmacology
Endoplasmic Reticulum / metabolism,  ultrastructure
Gap Junctions / chemistry,  metabolism,  ultrastructure
Golgi Apparatus / metabolism,  ultrastructure
Microscopy, Immunoelectron
Skin Neoplasms*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Actins; 0/Cadherins; 0/Connexin 43; 0/Connexins; 0/Culture Media; 127120-53-0/connexin 26; 7440-70-2/Calcium

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