Document Detail


Control of intercalation is cell-autonomous in the notochord of Ciona intestinalis.
MedLine Citation:
PMID:  12051819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dishevelled signaling plays a critical role in the control of cell intercalation during convergent extension in vertebrates. This study presents evidence that Dishevelled serves a similar function in the Ciona notochord. Embryos transgenic for mutant Dishevelled fail to elongate their tails, and notochord cells fail to intercalate, though notochord cell fates are unaffected. Analysis of mosaic transgenics revealed that the effects of mutant Dishevelled on notochord intercalation are cell-autonomous in Ciona, though such defects have nonautonomous effects in Xenopus. Furthermore, our data indicate that notochord cell intercalation in Ciona does not require the progressive signals which coordinate cell intercalation in the Xenopus notochord, highlighting an important difference in how mediolateral cell intercalation is controlled in the two animals. Finally, this study establishes the Ciona embryo as an effective in vivo system for the study of the molecular control of morphogenetic cell movements in chordates.
Authors:
David N Keys; Michael Levine; Richard M Harland; John B Wallingford
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  246     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-07     Completed Date:  2002-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-40     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, University of California, 401 Barker Hall, Berkeley, California 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Base Sequence
Ciona intestinalis / embryology*
DNA Primers
Morphogenesis
Mutation
Notochord / cytology*
Grant Support
ID/Acronym/Agency:
GM42341/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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