| Control of electroosmotic flow by a cation additive to enhance the separation of amino acids by micellar electrokinetic chromatography. | |
| | |
MedLine Citation:
|
PMID: 17418648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of a divalent cation (Mg2+) and 3 monovalent cations (Na+, Li+, and K+) as buffer additives on the electroosmotic flow (EOF) was investigated in order to improve the separation performance of p-acetamidobenzenesulfonyl fluoride (PAABS-F) derivatives of 20 standard amino acids by micellar electrokinetic chromatography (MEKC). The EOF can be decreased with increasing concentration of cations with the order of cations as Mg2+>K+>Na+>Li+. However, it was found that the resolution cannot be improved easily using a buffer cation which is more capable of decreasing the EOF. Taking the migration time, resolution, and peak shape into account, Na+ is the best buffer additive, although the EOF decreased most using Mg2+. Using 20 mmol/L borate at pH 9.3 containing 140 mmol/L SDS and 20 mmol/L Na+ as electrolyte, 20 standard amino acids were successfully separated within 14 min. |
| | |
Authors:
|
Qishu Qu; Xiaoqing Tang; Debby Mangelings; Chengyin Wang; Gongjun Yang; Xiaoya Hu; Chao Yan |
Related Documents
:
|
19224628 - Microcolumn lc enantioseparation of chiral compounds using diol silica gel functionaliz... 833228 - Quantitation of patulin pathway metabolites using gas-liquid chromatography. 11382288 - Determination of organic acids and inorganic anions in wine by isotachophoresis on a pl... 12498248 - Separation and enantioseparation of derivatized amino acids and biogenic amines by high... 1334228 - Aspirin, acetaminophen and proton transport through phospholipid bilayers and mitochond... 23163918 - Arenium acid-catalyzed deuteration of aromatic hydrocarbons. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-24 |
Journal Detail:
|
Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 853 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2007 Jun |
Date Detail:
|
Created Date: 2007-06-15 Completed Date: 2007-08-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 31-7 Citation Subset: IM |
Affiliation:
|
Jiangsu Key Laboratory of Environmental Materials and Environmental Engineering, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. quqishu@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
chemistry,
isolation & purification* Cations / chemistry* Chromatography, Micellar Electrokinetic Capillary / methods* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Cations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lung transplantation for cystic fibrosis: current concepts and one center's experience.
Next Document: Breath air analysis and its use as a biomarker in biological monitoring of occupational and environm...