Document Detail

Control of the distribution of hemidesmosome components in cultured keratinocytes: Ca2+ and phorbol esters.
MedLine Citation:
PMID:  1284069     Owner:  NLM     Status:  MEDLINE    
DJM-1 cells (a human squamous cell carcinoma cell line) grown in low Ca2+ medium did not form cell-cell junctions of desmosome-keratin intermediate filament (KIF). When they were shifted to normal (high) Ca2+ medium, rapid translocation of desmoplakins from the cytosol to the plasma membrane to form desmosomes and reorganization of 180 kd-hemidesmosome proteins were induced almost simultaneously. In correlation with these morphological responses, the Ca2+ shift caused a breakdown of inositol phospholipids, a formation of diacylglycerol (DAG) and inositol trisphosphate (IP3), protein kinase C (PKC) activation, and Ca2+ influx. 12-0-tetradecanoylphorbol-13-acetate (TPA)-treatment of low Ca(2+)-grown DJM-1 cells also caused desmosome formation in association with PKC activation. These TPA effects were cancelled with PKC inhibitors, 1-(5-isoquinolinylsulfomyl)-2-methylpiperazine (H7) and staurosporine. Treatment with other PKC-activating agents, phorbol-12,13-butyrate (PDBu) and diaoctanoylglycerol (DOG), also induced desmosome formation. TPA-treatment of normal Ca(2+)-grown cells collapsed the organized distribution of the 180 kd-hemidesmosome protein and appeared to detach this protein from the cell-matrix adhering sites. This effect was also inhibited by H7. These results suggest that PKC activation plays important roles in upregulation of cell-cell junctions and downregulation of cell-matrix junctions in association with differentiation of keratinocytes.
Y Kitajima; K Owaribe; Y Nishizawa; H Yaoita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of dermatology     Volume:  19     ISSN:  0385-2407     ISO Abbreviation:  J. Dermatol.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-04-05     Completed Date:  1993-04-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7600545     Medline TA:  J Dermatol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  770-3     Citation Subset:  IM    
Department of Dermatology, Jichi Medical School, Tochigi, Japan.
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MeSH Terms
Calcium / pharmacology*
Desmosomes / physiology*,  ultrastructure
Enzyme Activation
Intercellular Junctions / physiology
Keratinocytes / metabolism,  ultrastructure*
Keratins / physiology
Phosphatidylinositols / metabolism
Protein Kinase C / metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Reg. No./Substance:
0/Phosphatidylinositols; 16561-29-8/Tetradecanoylphorbol Acetate; 68238-35-7/Keratins; 7440-70-2/Calcium; EC Kinase C

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