| Control of cytoplasmic calcium with photolabile tetracarboxylate 2-nitrobenzhydrol chelators. | |
| | |
MedLine Citation:
|
PMID: 3098316 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This paper introduces nitr-2, a new Ca2+ chelator designed to release Ca2+ upon illumination with near UV (300-400 nm) light. Before illumination nitr-2 has Ca2+ dissociation constants of 160 and 630 nM in 0.1 and 0.3 M ionic strength respectively; after photoconversion to a nitrosobenzophenone the values shift to 7 and 18 microM, high enough to liberate substantial amounts of Ca2+ under intracellular conditions. The speed of release is limited by a dark reaction with rate constant 5 s-1. Aplysia central neurons injected with nitr-2 and exposed to UV light exhibit two separate Ca2+-dependent membrane currents: one carried by potassium ions and one a nonspecific cation current. A quantitative estimate of the spatial distribution of intracellular [Ca2+] changes in large cells filled with a high concentration of nitr-2 and exposed to an intense UV flash is offered. |
| | |
Authors:
|
R Y Tsien; R S Zucker |
Related Documents
:
|
8006956 - Role of calcium in the modulation of vicia guard cell potassium channels by abscisic ac... 9548666 - Ca2+ signaling in the pancreatic acinus. 8382056 - A steady-state mechanism can account for the properties of inositol 2,4,5-trisphosphate... 8653756 - Calcium signalling in granule neurones studied in cerebellar slices. 8006956 - Role of calcium in the modulation of vicia guard cell potassium channels by abscisic ac... 23470626 - Lipoxin a4 activates alveolar epithelial enac, na,k-atpase and increases alveolar fluid... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biophysical journal Volume: 50 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1986 Nov |
Date Detail:
|
Created Date: 1987-02-11 Completed Date: 1987-02-11 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 843-53 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aplysia Calcium / metabolism* Chelating Agents / pharmacology* Cytoplasm / metabolism Egtazic Acid / analogs & derivatives*, chemical synthesis, pharmacology Kinetics Membrane Potentials / drug effects Neurons / physiology Photolysis |
| Grant Support | |
ID/Acronym/Agency:
|
EY-04372/EY/NEI NIH HHS; GM-31004/GM/NIGMS NIH HHS; NS-15114/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chelating Agents; 103471-64-3/nitr 2; 67-42-5/Egtazic Acid; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inactivation of follicle-stimulating hormone by a factor in a bovine serum albumin preparation.
Next Document: In vitro immune interferon induction during tumor evolution.