Document Detail


Control of cytokine gene expression by PG101, a water-soluble extract prepared from Lentinus lepideus.
MedLine Citation:
PMID:  16344041     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A water-soluble extract from Lentinus lepideus mycelium, named PG101, has been shown to control the expression of various cytokines [M. Jin, H.J. Jung, J.J. Choi, H.Jeon, J.H. Oh, B. Kim, S.S. Shin, J.K. Lee, K. Yoon, S. Kim, Activation of selective transcription factors and cytokines by water-soluble extract from Lentinus lepideus, Exp. Biol. Med. (Maywood) 228 (2003) 749-758]. To understand its molecular mechanism(s), PG101-mediated activation of cytokines was studied at the RNA and protein levels. Results from Northern blot analysis indicated that the steady-state RNA levels of TNF-alpha and seven other cytokines were highly increased in human peripheral blood mononuclear cells treated with PG101. The RNA level of TNF-alpha, MIP-1alpha, MIP-1beta, MIP-3alpha, and IL-8 was not affected by the presence of cycloheximide, an inhibitor of the translation process, suggesting that they are the direct targets of PG101. A significantly high protein level of TNF-alpha, MIP-1alpha, and IL-8 remained detectable, even when cells were cultured with actinomycin D, 2h prior to the PG101 treatment. Our data indicate that PG101 controls selective cellular proteins, which play key roles in the innate immune system, at the transcriptional and post-translational levels.
Authors:
Jeong June Choi; Mirim Jin; Jong Kyu Lee; Wi Young Lee; Yong-il Park; Yong Nam Han; Sunyoung Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  339     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-13     Completed Date:  2006-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  880-7     Citation Subset:  IM    
Affiliation:
School of Biological Science, Seoul National University, Seoul 151-742, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Biological Products / administration & dosage*,  chemistry
Cytokines / immunology*
Dose-Response Relationship, Drug
Gene Expression Regulation / drug effects,  immunology*
Humans
Lentinula / chemistry*
Leukocytes, Mononuclear / drug effects,  immunology*
Solubility
Water / chemistry
Chemical
Reg. No./Substance:
0/Biological Products; 0/Cytokines; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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