| Control of blood pressure variability in caveolin-1-deficient mice: role of nitric oxide identified in vivo through spectral analysis. | |
| | |
MedLine Citation:
|
PMID: 18349137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIMS: In endothelial cells, caveolin-1 (cav-1) is known to negatively modulate the activation of endothelial nitric oxide synthase, a key regulator of blood pressure (BP). However, the impact of genetic alteration of cav-1 on vascular nitric oxide (NO) production and BP homeostasis in vivo is unknown. METHODS AND RESULTS: We used spectral analysis of systolic blood pressure (SBP) variability in mice chronically equipped with telemetry implants to identify frequency ranges (0.05-0.4 Hz; very low frequency, VLF) specifically responding to NO, independently of changes in absolute BP or systemic neurohormone levels. VLF variability was inversely correlated to aortic vasodilator-stimulated Ser(239) phosphoprotein (VASP) phosphorylation, reflecting NO bioactivity. We show that mice deficient in cav-1 have decreased VLF variability paralleled with enhanced systemic and vascular production of NO at unchanged mean SBP levels. Conversely, VLF variability was increased upon acute injection of mice, with a peptide containing the caveolin-scaffolding domain (CSD; residues 82-101) fused to an internalization sequence of antennapedia that decreased vascular and circulating NO in vivo. CONCLUSION: These data highlight the functional importance of cav-1 for the production of bioactive NO in conduit arteries and its control of central BP variability. Given the impact of the latter on target organ damage, this raises the interest for genetic, pharmacological, or molecular interventions that modulate cav-1 expression in diseases with NO-dependent endothelial dysfunction. |
| | |
Authors:
|
Fanny Desjardins; Irina Lobysheva; Michel Pelat; Bernard Gallez; Olivier Feron; Chantal Dessy; Jean-Luc Balligand |
Related Documents
:
|
17437657 - Endogenous nitric oxide reduces the efficacy of the endothelin system to maintain blood... 1619907 - Evaluation of endothelium-derived nitric oxide mediated vasodilation utilizing ex vivo ... 9369347 - Inhibition of nitric oxide synthase and soluble guanylate cyclase induces cardiodepress... 8836907 - Portal vascular responsiveness to sympathetic stimulation and nitric oxide in cirrhotic... 11497197 - Association of increased pulse pressure with the development of heart failure in shep. ... 23296267 - Distribution and abundance of white-fronted spider monkeys, ateles belzebuth (atelidae)... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-18 |
Journal Detail:
|
Title: Cardiovascular research Volume: 79 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-07-30 Completed Date: 2008-12-02 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 527-36 Citation Subset: IM |
Affiliation:
|
Unit of Pharmacology and Therapeutics, Université catholique de Louvain, UCL-FATH 5349, Vésale+5, 52 Avenue Mounier, 1200 Brussels, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aorta / metabolism Blood Pressure* / drug effects, genetics Blood Pressure Monitoring, Ambulatory / methods* Cattle Caveolin 1 / deficiency, genetics, metabolism* Cells, Cultured Circadian Rhythm* Dose-Response Relationship, Drug Electrocardiography, Ambulatory Electron Spin Resonance Spectroscopy Endothelial Cells / drug effects, enzymology, metabolism* Enzyme Inhibitors / pharmacology Fourier Analysis* Heart Rate Male Mice Mice, Inbred C57BL Mice, Knockout NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide / blood* Nitric Oxide Donors / pharmacology Nitric Oxide Synthase Type II / antagonists & inhibitors, metabolism Nitric Oxide Synthase Type III Nitroprusside / pharmacology Peptide Fragments / pharmacology Signal Processing, Computer-Assisted Telemetry* |
| Chemical | |
Reg. No./Substance:
|
0/Cav1 protein, mouse; 0/Caveolin 1; 0/Enzyme Inhibitors; 0/Nitric Oxide Donors; 0/Peptide Fragments; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vascular repair by endothelial progenitor cells.
Next Document: FK506 can activate transforming growth factor-beta signalling in vascular smooth muscle cells and pr...