Document Detail


Control of antigen presentation with a photoreleasable agonist peptide.
MedLine Citation:
PMID:  17131984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IEk major histocompatibility complex (IEk), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IEk did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ.
Authors:
Andrew L DeMond; Toby Starr; Michael L Dustin; Jay T Groves
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  128     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-29     Completed Date:  2007-02-01     Revised Date:  2008-01-17    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15354-5     Citation Subset:  IM    
Affiliation:
Biophysics Graduate Group and Department of Chemistry, University of California, Berkeley, California 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation / immunology*
Calcium Signaling
Lipid Bilayers
Lymphocyte Activation / immunology*
Major Histocompatibility Complex / immunology
Peptide Fragments / immunology*
Photochemistry
Receptors, Antigen, T-Cell / immunology
T-Lymphocytes / immunology*,  radiation effects
Ultraviolet Rays
Grant Support
ID/Acronym/Agency:
AI044931/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Peptide Fragments; 0/Receptors, Antigen, T-Cell

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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