| Control of antigen presentation with a photoreleasable agonist peptide. | |
| | |
MedLine Citation:
|
PMID: 17131984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IEk major histocompatibility complex (IEk), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IEk did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ. |
| | |
Authors:
|
Andrew L DeMond; Toby Starr; Michael L Dustin; Jay T Groves |
Related Documents
:
|
21428684 - A finite element model of cell-matrix interactions to study the differential effect of ... 7642714 - Transfected connexin45 alters gap junction permeability in cells expressing endogenous ... 6696964 - Gap junction modulation in rat uterus. i. effects of estrogens on myometrial and serosa... 3933364 - Some electrical and pharmacological properties of gap junctions between adult ventricul... 2752214 - Cell cycle-specific variation of intracellular plasminogen activator activity in cultur... 22936984 - The b-myb transcriptional network guides cell cycle progression and fate decisions to s... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of the American Chemical Society Volume: 128 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-11-29 Completed Date: 2007-02-01 Revised Date: 2008-01-17 |
Medline Journal Info:
|
Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
|
Languages: eng Pagination: 15354-5 Citation Subset: IM |
Affiliation:
|
Biophysics Graduate Group and Department of Chemistry, University of California, Berkeley, California 94720, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigen Presentation / immunology* Calcium Signaling Lipid Bilayers Lymphocyte Activation / immunology* Major Histocompatibility Complex / immunology Peptide Fragments / immunology* Photochemistry Receptors, Antigen, T-Cell / immunology T-Lymphocytes / immunology*, radiation effects Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
|
AI044931/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Lipid Bilayers; 0/Peptide Fragments; 0/Receptors, Antigen, T-Cell |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of intercessory prayer on wound healing in nonhuman primates.
Next Document: Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-co...