Document Detail

Control of antigen presentation with a photoreleasable agonist peptide.
MedLine Citation:
PMID:  17131984     Owner:  NLM     Status:  MEDLINE    
The immunological synapse is a specialized intercellular junction between a T cell and a target cell that orchestrates the engagement of receptors and ligands in space and time as a means of regulating function. Here we introduce a reagent for controlling the spatial and temporal presentation of natural antigen to T cells. Moth cytochrome c (88-103) peptide (MCC), an agonist to the murine T cell receptor AND when presented in the context of H2 IEk major histocompatibility complex (IEk), was synthesized with the side-chain amine of Lys99 conjugated to a photosensitive protecting group, 6-nitroveratryloxycarbonyl (NVOC). Cells plated on supported bilayers displaying mobile intercellular adhesion molecule-1 (ICAM-1) and NVOC-MCC loaded IEk did not form immunological synapses and exhibited low intracellular calcium levels, similar to cells presented with self-peptide. Irradiation with UV light was sufficient to restore agonist activity in situ.
Andrew L DeMond; Toby Starr; Michael L Dustin; Jay T Groves
Related Documents :
7464934 - Induction of cell--cell channel formation by mrna.
8759104 - Atrioventricular junctional tissue. discrepancy between histological and electrophysiol...
18825214 - Improvement of conversion efficiency for multi-junction solar cells by incorporation of...
7537274 - Specific permeability and selective formation of gap junction channels in connexin-tran...
8912374 - Microdosimetry and radiocurability: modelling targeted therapy with beta-emitters.
9450634 - Cytoskeletal characterization of arteriovenous epithelioid cells.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  128     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-29     Completed Date:  2007-02-01     Revised Date:  2008-01-17    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15354-5     Citation Subset:  IM    
Biophysics Graduate Group and Department of Chemistry, University of California, Berkeley, California 94720, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigen Presentation / immunology*
Calcium Signaling
Lipid Bilayers
Lymphocyte Activation / immunology*
Major Histocompatibility Complex / immunology
Peptide Fragments / immunology*
Receptors, Antigen, T-Cell / immunology
T-Lymphocytes / immunology*,  radiation effects
Ultraviolet Rays
Grant Support
Reg. No./Substance:
0/Lipid Bilayers; 0/Peptide Fragments; 0/Receptors, Antigen, T-Cell

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effect of intercessory prayer on wound healing in nonhuman primates.
Next Document:  Activity-based substrate profiling for Gcn5-related N-acetyltransferases: the use of chloroacetyl-co...