Document Detail


Control of Plasmodium falciparum erythrocytic cycle: gamma-delta T cells target the red blood cell-invasive merozoites.
MedLine Citation:
PMID:  22045985     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The control of Plasmodium falciparum erythrocytic parasite density is essential for protection against malaria, as it prevents pathogenesis and progression towards severe disease. P.falciparum blood-stage parasite cultures are inhibited by human Vγ9Vδ2 gamma-delta T cells, but the underlying mechanism remains poorly understood. Here, we show that both intra-erythrocytic parasites and the extracellular red blood cell-invasive merozoites specifically activate Vγ9Vδ2 T cells in a γδ T cell receptor dependent manner and trigger their degranulation. In contrast, the γδ T cell-mediated anti-parasitic activity only targets the extracellular merozoites. Using perforin-deficient and granulysin-silenced T cell lines, we demonstrate that granulysin is essential for the in vitro anti-plasmodial process, whereas perforin is dispensable. Patients infected with P.falciparum exhibited elevated granulysin plasma levels associated with high levels of granulysin-expressing Vδ2(+) T cells endowed with parasite-specific degranulation capacity. This indicates in vivo activation of Vγ9Vδ2 T cells along with granulysin triggering and discharge during primary acute falciparum malaria. Altogether, this work identifies Vγ9Vδ2 T cells as unconventional immune effectors targeting the red blood cell-invasive extracellular P.falciparum merozoites and opens novel perspectives for immune interventions harnessing the anti-parasitic activity of Vγ9Vδ2 T cells to control parasite density in malaria patients.
Authors:
Giulia Costa; Séverine Loizon; Marianne Guenot; Iulia Mocan; Franck Halary; Geneviève de Saint-Basile; Vincent Pitard; Julie Déchanet-Merville; Jean-François Moreau; Marita Troye-Blomberg; Odile Mercereau-Puijalon; Charlotte Behr
Related Documents :
21791455 - Factors responsible for deep-sowing tolerance in wheat seedlings: varietal differences ...
22919565 - Protection by glia-conditioned medium in a cell model of huntington disease.
22226525 - Methods in cell biology: analysis of cell polarity in c. elegans embryos.
22528485 - Transcellular neuroligin-2 interactions enhance insulin secretion and are integral to p...
3955665 - A heat shock-resistant variant of chinese hamster cell line constitutively expressing h...
15122585 - Overexpression of cdk4/cyclin d1, a possible mediator of apoptosis and an indicator of ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-1
Journal Detail:
Title:  Blood     Volume:  -     ISSN:  1528-0020     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
CNRS, UMR 5164, Bordeaux, France;
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Megakaryocyte-specific RhoA deficiency causes macrothrombocytopenia and defective platelet activatio...
Next Document:  In situ vaccination against mycosis fungoides by intratumoral injection of a TLR9 agonist combined w...