Document Detail


Control of neural cell composition in poly(ethylene glycol) hydrogel culture with soluble factors.
MedLine Citation:
PMID:  21823990     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(ethylene glycol) (PEG) hydrogels are being developed as cell delivery vehicles that have great potential to improve neuronal replacement therapies. Current research priorities include (1) characterizing neural cell growth within PEG hydrogels relative to standard culture systems and (2) generating neuronal-enriched populations within the PEG hydrogel environment. This study compares the percentage of neural precursor cells (NPCs), neurons, and glia present when dissociated neural cells are seeded within PEG hydrogels relative to standard monolayer culture. Results demonstrate that PEG hydrogels enriched the initial cell population for NPCs, which subsequently gave rise to neurons, then to glia. Relative to monolayer culture, PEG hydrogels maintained an increased percentage of NPCs and a decreased percentage of glia. This neurogenic advantage of PEG hydrogels is accentuated in the presence of basic fibroblast growth factor and epidermal growth factor, which more potently increase NPC and neuronal expression markers when applied to cells cultured within PEG hydrogels. Finally, this work demonstrates that glial differentiation can be selectively eliminated upon supplementation with a γ-secretase inhibitor. Together, this study furthers our understanding of how the PEG hydrogel environment influences neural cell composition and also describes select soluble factors that are useful in generating neuronal-enriched populations within the PEG hydrogel environment.
Authors:
Rachael Mooney; Sarah Haeger; Rasheed Lawal; Mariah Mason; Neha Shrestha; Alexander Laperle; Kimberly Bjugstad; Melissa Mahoney
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-08-08
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  17     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-01     Completed Date:  2012-05-29     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2805-15     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Colorado, Boulder, Colorado 80309, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects,  genetics
Cells, Cultured
Dipeptides / chemistry,  pharmacology
Epidermal Growth Factor / chemistry,  pharmacology
Fibroblast Growth Factor 2 / chemistry,  pharmacology
Hydrogel / chemistry*
Immunohistochemistry
Neurons / cytology*,  drug effects*
Polyethylene Glycols / chemistry*
Rats
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
R01 NS052597-02/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Dipeptides; 0/N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; 0/Polyethylene Glycols; 103107-01-3/Fibroblast Growth Factor 2; 25852-47-5/Hydrogel; 62229-50-9/Epidermal Growth Factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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