| Control of Ca2+ wave propagation in mouse pancreatic acinar cells. | |
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MedLine Citation:
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PMID: 9530097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated control mechanisms involved in the propagation of agonist-induced Ca2+ waves in isolated mouse pancreatic acinar cells. Using a confocal laser-scanning microscope, we were able to show that maximal stimulation of cells with acetylcholine (ACh, 500 nM) or bombesin (1 nM) caused an initial Ca2+ release of comparable amounts with both agonists at the luminal cell pole. Subsequent Ca2+ spreading to the basolateral membrane was faster with ACh (17.3 +/- 5.4 microns/s) than with bombesin (8.0 +/- 2.2 microns/s). The speed of bombesin-induced Ca2+ waves could be increased up to the speed of ACh-induced Ca2+ waves by inhibition of protein kinase C (PKC). Activation of PKC significantly decreased the speed of ACh-induced Ca2+ waves but had only little effect on bombesin-evoked Ca2+ waves. Within 3 s after stimulation, production of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was higher in the presence of ACh compared with bombesin, whereas bombesin induced higher levels of diacylglycerol (DAG) than ACh. These data suggest that the slower propagation speed of bombesin-induced Ca2+ waves is due to higher activation of PKC in the presence of bombesin compared with ACh. The higher increase in bombesin-compared with ACh-induced DAG production is probably due to activation of phospholipase D (PLD). Inhibition of the PLD-dependent DAG production by preincubation with 0.3% butanol led to an acceleration of the bombesin-induced Ca2+ wave. In further experiments, we could show that ruthenium red (100 microM), an inhibitor of Ca(2+)-induced Ca2+ release in skeletal muscle, also decreased the speed of ACh-induced Ca2+ waves. The effect of ruthenium red was not additive to the effect of PKC activation. From the data, we conclude that, following Ins(1,4,5)P3-induced Ca2+ release in the luminal cell pole, secondary Ca2+ release from stores, which are located in series between the luminal and the basal plasma membrane, modifies Ca2+ spreading toward the basolateral cell side by Ca(2+)-induced Ca2+ release. Activation of PKC leads to a reduction in Ca2+ release from these stores and therefore could explain the slower propagation of Ca2+ waves in the presence of bombesin compared with ACh. |
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Authors:
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F Pfeiffer; L Sternfeld; A Schmid; I Schulz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 274 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Mar |
Date Detail:
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Created Date: 1998-04-24 Completed Date: 1998-04-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C663-72 Citation Subset: IM |
Affiliation:
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Institute of Physiology II, University of the Saarland, Homburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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metabolism Animals Bombesin / pharmacology Calcium / metabolism* Calcium Channels / drug effects, metabolism Calcium-Transporting ATPases / antagonists & inhibitors Endoplasmic Reticulum / drug effects, enzymology Enzyme Activation Enzyme Inhibitors / pharmacology Hydroquinones / pharmacology Indicators and Reagents / pharmacology Inositol 1,4,5-Trisphosphate / metabolism Male Mice Pancreatic Neoplasms / metabolism* Periodicity Phosphatidylinositol 4,5-Diphosphate / metabolism Protein Kinase C / antagonists & inhibitors Ruthenium Red / pharmacology Signal Transduction Type C Phospholipases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channels; 0/Enzyme Inhibitors; 0/Hydroquinones; 0/Indicators and Reagents; 0/Phosphatidylinositol 4,5-Diphosphate; 11103-72-3/Ruthenium Red; 1948-33-0/2-tert-butylhydroquinone; 31362-50-2/Bombesin; 51-84-3/Acetylcholine; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 2.7.11.13/Protein Kinase C; EC 3.1.4.-/Type C Phospholipases; EC 3.6.1.8/Calcium-Transporting ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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