Document Detail


Contribution to the study of the enzymatic profiles of yeast organisms with medical interest.
MedLine Citation:
PMID:  6310400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The enzymatic profiles of several yeastlike organisms were studied using 19 substrates included in the API ZYM system. The isolates evaluated were: 186 Candida albicans, 19 C. stellatoidea, 4 C. tropicalis, 2 C. parapsilosis, 2 C. pseudotropicalis, 1 C. guilliermondii, 3 C. krusei, 11 Torulopsis (Candida) glabrata, 1 Cryptococcus neoformans, 2 Saccharomyces carlsbergensis, 1 Rhodotorula rubra, and 1 R. mucilaginosa. Esterase lipase (C8), leucine arylamidase, acid phosphatase, and phosphoamidase were detected in all of the isolates while trypsin and alpha-galactosidase were not found in any of the isolates using this system. The other enzymes were produced to a variable degree. The different enzymatic profiles might prove useful in the rapid differential diagnosis of genera and species of these yeastlike organisms. To this end, more extensive studies using more isolates of each species will be required, and enzymatic activity should be verified with other techniques and substrates.
Authors:
M Casal; M J Linares
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Mycopathologia     Volume:  81     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  1983 Mar 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aminopeptidases / metabolism
Candida / enzymology*
Candida albicans / enzymology
Cryptococcus / enzymology*
Cryptococcus neoformans / enzymology*
Glycoside Hydrolases / metabolism
Hydrolases / metabolism*
Lipase / metabolism
Mitosporic Fungi / enzymology*
Peptide Hydrolases / metabolism
Phosphoric Monoester Hydrolases / metabolism
Rhodotorula / enzymology*
Saccharomyces / enzymology*
Species Specificity
Chemical
Reg. No./Substance:
EC 3.-/Hydrolases; EC 3.1.1.3/Lipase; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.4.-/Peptide Hydrolases; EC 3.4.11.-/Aminopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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