| Contribution of several amino acids and lactate to gluconeogenesis in hepatocytes isolated from rats fed various diets. | |
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MedLine Citation:
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PMID: 2303909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The contribution under various nutritional regimens of several amino acids and lactate to gluconeogenesis was estimated by measuring the glucose formation from 14C-labeled substrates. Isolated rat hepatocytes were incubated for 60 min in a Krebs-Ringer bicarbonate buffer pH 7.4 containing lactate, pyruvate and all the amino acids at concentrations similar to their physiological levels found in rat plasma, with one precursor labeled in each flask. In all conditions, lactate was the major glucose precursor, providing over 60% of the glucose formed. Glutamine and alanine were the major amino acid precursors of glucose, contributing 9.8% and 10.6% of the glucose formed, respectively, in hepatocytes isolated from starved rats. Serine, glycine and threonine also contributed to gluconeogenesis in the starved liver cells at 2.6, 2.1 and 3.8%, respectively, of the glucose formed. The rate of glucose formation from the isolated hepatocytes of the starved rats and those fed either high protein or high fat was higher than that from rats fed a nonpurified diet. |
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Authors:
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M Kaloyianni; R A Freedland |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of nutrition Volume: 120 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 1990 Jan |
Date Detail:
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Created Date: 1990-03-21 Completed Date: 1990-03-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 116-22 Citation Subset: IM |
Affiliation:
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Department of Physiological Science, Veterinary Medicine School, University of California, Davis 95616. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Analysis of Variance Animals Carbon Radioisotopes Dietary Fats / administration & dosage*, metabolism Dietary Proteins / administration & dosage*, metabolism Female Food Deprivation / physiology Gluconeogenesis* / physiology Glucose / metabolism Hydrogen-Ion Concentration Lactates / metabolism* Lactic Acid Liver / metabolism* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Radioisotopes; 0/Dietary Fats; 0/Dietary Proteins; 0/Lactates; 50-21-5/Lactic Acid; 50-99-7/Glucose |
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