Document Detail

Contribution of scanning electron microscopy to viewing internal cell structure.
MedLine Citation:
PMID:  6762649     Owner:  NLM     Status:  MEDLINE    
Freeze-fracture provides a way of opening up cells and tissues for an internal view of cytoplasm and nucleus, and can give an internal view of a cytoplasmic organelle if the plane of fracture cuts through the organelle. Selective removal of soluble or other components is necessary for a deep view of structure at the fracture face. This may be achieved by osmium digestion, or by glycerol extraction, or by delaying fixation until after freeze-fracture and thawing, or by prior treatment with detergent to remove cell membranes and wash out soluble components. Cells may also be ruptured at room temperature, or in certain cases prepared to expose the inner surface of the plasma membrane for scanning electron microscopy (SEM) viewing. The problems and potential of SEM viewing of the cell interior are in certain respects similar to those encountered and derived from TEM replica study of freeze-fractured cells after deep etching. TEM replicas give better resolution, while SEM offers advantages in study of surfaces with considerable depth of structure. Study of fresh material, without fixation or alcohol dehydration, by rapid freezing and deep etching, is increasing our understanding of the artifacts that can be produced by these two preparative steps which are essential to critical point drying, whether used as a preparative step for SEM or for whole-mount high-voltage TEM microscopy.
G H Haggis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scanning electron microscopy     Volume:  -     ISSN:  0586-5581     ISO Abbreviation:  Scan Electron Microsc     Publication Date:  1982  
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0371617     Medline TA:  Scan Electron Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  751-63     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / ultrastructure
Cells / ultrastructure*
Cells, Cultured
Chromosomes / ultrastructure
Escherichia coli / ultrastructure
Freeze Fracturing / methods
Lymphocyte Activation
Lymphocytes / ultrastructure
Microscopy, Electron, Scanning / methods
Proteins / analysis
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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