| Contribution of phosphoinositides and phosphatidylcholines to the production of phosphatidic acid upon concanavalin A stimulation of rat thymocytes. | |
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MedLine Citation:
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PMID: 8906587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stimulation of rat thymocytes by concanavalin A (Con A) results in a very early increase of the cellular level of phosphatidic acid (PA), while that of diacylglycerol (DAG) was not affected. As the biological activity of PA is very likely to be determined by its molecular species composition, the present study aims to investigate the pathways leading to the production of PA in Con A-stimulated rat thymocytes. Prelabeling the cells with [3H]arachidonic acid, [3H]myristic acid, [3H]choline, or [14C]lysophosphatidylcholine allowed us to determine that PA is formed by both phosphoinositide (PIs) and phosphatidylcholine (PC) hydrolysis. We then investigated whether PA derived from PC was formed by phospholipase C (PLC) or phospholipase D (PLD) hydrolysis. In the presence of 1-butanol, the production of phosphatidylbutanol was only observed in tetradecanoyl phorbol acetate (TPA)-stimulated cells. The use of a specific PC phospholipase C inhibitor resulted in a decrease of Con A-stimulated PA production in cells labeled with [3H]myristate. When cells were labeled with [3H]choline, only TPA stimulation induced a release of labeled choline. All together, these experiments suggest that PA is originated from two phospholipid sources, predominantly PI via PLC hydrolysis and to a lesser extent PC, by PLC hydrolysis also. Molecular species analyses by reverse phase HPLC are in agreement with this hypothesis, as diacyl-GP molecular species composition is similar to that of diacyl-GPC and DAG in resting cells, but resembles that of diacyl-GPI in Con A-treated cells. Thus, in stimulated cells, the amount of 18:0/20:4 species doubled while those of saturated and monounsaturated species decreased. |
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Authors:
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S el Bawab; O Macovschi; C Thevenon; A Goncalves; G Némoz; M Lagarde; A F Prigent |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of lipid research Volume: 37 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1997-03-04 Completed Date: 1997-03-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2098-108 Citation Subset: IM |
Affiliation:
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INSERM Unité 352, Laboratoire de Biochimie et Pharmacologie INSA-Lyon, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism Concanavalin A / pharmacology* Diglycerides / biosynthesis Hydrolysis Male Phosphatidic Acids / biosynthesis* Phosphatidylcholines / metabolism* Phosphatidylinositols / metabolism* Phospholipase D / metabolism Rats Rats, Sprague-Dawley Tetradecanoylphorbol Acetate / pharmacology Thymus Gland / drug effects*, metabolism* Tritium Type C Phospholipases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phosphatidylinositols; 10028-17-8/Tritium; 11028-71-0/Concanavalin A; 16561-29-8/Tetradecanoylphorbol Acetate; 506-32-1/Arachidonic Acid; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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