| Contribution of mitochondrial proton leak to skeletal muscle respiration and to standard metabolic rate. | |
| | |
MedLine Citation:
|
PMID: 8897845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have tested the hypothesis that the leak of protons across the mitochondrial inner membrane (proton leak) is a significant contributor to standard metabolic rate (SMR). We found that proton leak accounts for around one-half of the resting respiration rate of perfused rat skeletal muscle. Proton leak is known to make a significant (26%) contribution to the resting respiration rate of isolated rat hepatocytes (M. D. Brand, L.-F. Chien, E. K. Ainscow, D. F. S. Rolfe, and R. K. Porter. Biochim. Biophys. Acta 1187: 132-139, 1994). If the importance of proton leak in these isolated and perfused systems is similar to its importance in vivo, then using literature values for the contribution of liver and skeletal muscle to SMR, we can calculate that proton leak in liver and skeletal muscle alone accounts for 11-26% (mean 20%) of the SMR of the rat. If proton leak activity in the other tissues of the rat is similar to that in liver cells, then the contribution of proton leak to rat SMR would be 16-31% (mean 25%). |
| | |
Authors:
|
D F Rolfe; M D Brand |
Related Documents
:
|
20564335 - Poloxamer 407 increases the recovery of paracetamol in the isolated perfused rat liver. 696875 - Metabolism of arginine by the isolated perfused rat kidney. 3355595 - Synergistic effects of hypoxia and fasting on harmol elimination in the isolated perfus... 22294125 - Hepatic clearance and drug metabolism using isolated perfused rat liver. 4058425 - A new nucleosomal protein in normal liver related to the cytoplasmic polypeptide target... 6466295 - Kynurenine metabolism in vitamin-b-6-deficient rat liver after tryptophan injection. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Oct |
Date Detail:
|
Created Date: 1996-12-16 Completed Date: 1996-12-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: C1380-9 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, University of Cambridge, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
metabolism Animals Electric Conductivity Energy Metabolism Female Hydrogen-Ion Concentration Intracellular Membranes / physiology Membrane Potentials Mitochondria, Muscle / metabolism* Muscle Proteins / biosynthesis Muscle, Skeletal / metabolism* Oligomycins / pharmacology Oxygen Consumption Pentobarbital / pharmacology Permeability Rats Rats, Wistar Sarcolemma / physiology Uncoupling Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Muscle Proteins; 0/Oligomycins; 0/Uncoupling Agents; 56-65-5/Adenosine Triphosphate; 76-74-4/Pentobarbital |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Membrane domain localization of pH-regulating transporters in frog skeletal muscle membrane vesicles...
Next Document: Modeling spiral Ca2+ waves in single cardiac cells: role of the spatial heterogeneity created by the...