Document Detail

Contribution of immunohistochemistry, electron microscopy, and cell culture to the characterization of nonfunctioning pituitary adenomas: a study of 40 cases.
MedLine Citation:
PMID:  1468769     Owner:  NLM     Status:  MEDLINE    
We studied 40 endocrinologically inactive pituitary adenomas by immunohistochemistry, electron microscopy, and cell culture in order to determine the incidence of gonadotropic adenomas and to classify nonfunctioning adenomas. Immunohistochemical studies using a large panel of monoclonal and polyclonal antibodies identified the following nonfunctioning adenomas: 20 gonadotropic adenomas, four silent corticotropic adenomas, one plurihormonal adenoma, and 15 nonsecreting adenomas. Among nonsecreting adenomas, ultrastructural study of 13 cases identified seven null cell adenomas and six oncocytomas. Silent corticotropic adenomas were classified into subtypes I, II, and III according to Kovacs and Horvath. Most often, gonadotropic adenomas displayed a varying number of oncocytic cells, characteristic secretory granules, and a prominent Golgi apparatus. Postembedding immunoelectron microscopy was performed on eight gonadotropic or nonsecreting adenomas, but this technique did not provide any additional information. Six gonadotropic adenomas and 10 so-called nonsecreting adenomas were studied in primary cell cultures. The six gonadotropic adenomas and seven of the 10 nonsecreting adenomas released gonadotropins in the culture medium. The use of in vitro results as a supplementary diagnostic criterion allowed classification of the 40 nonfunctioning adenomas as follows: 27 gonadotropic adenomas, four silent corticotropic adenomas, one plurihormonal adenoma, and eight nonsecreting adenomas. These results demonstrate a high proportion of gonadotropic adenomas among nonfunctioning adenomas (67.5%) and the usefulness of several techniques in characterizing this type of pituitary adenoma.
A Croue; V Beldent; M C Rousselet; G Guy; V Rohmer; J C Bigorgne; J P Saint-Andre
Related Documents :
20472479 - Human growth hormone: 45-kda isoform with extraordinarily stable interchain disulfide l...
15647919 - Colocalization of gh, tsh and prolactin, but not acth, with betalh-immunoreactivity: ev...
2491809 - Growth hormone (gh) response to gh-releasing hormone by perifused pituitary cells from ...
9099899 - Gh3 cells expressing constitutively active gs alpha (q227l) show enhanced hormone secre...
10720059 - A human pituitary tumor-derived folliculostellate cell line.
2993719 - Sources of extramitochondrial corticoidogenic cholesterol in the adrenal cortex.
17662039 - Trypanosoma brucei polo-like kinase is essential for basal body duplication, kdna segre...
19947169 - Reversal of p-glycoprotein-mediated multidrug resistance by guggulsterone in doxorubici...
22419889 - Prognostic values of filamin-a status for topoisomerase ii poison chemotherapy.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human pathology     Volume:  23     ISSN:  0046-8177     ISO Abbreviation:  Hum. Pathol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-27     Completed Date:  1993-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421547     Medline TA:  Hum Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1332-9     Citation Subset:  IM    
Department of Endocrinology, Centre Hospitalier Régional et Universitaire, Angers, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenoma / chemistry,  pathology*,  ultrastructure
Chorionic Gonadotropin / analysis
Follicle Stimulating Hormone / analysis
Luteinizing Hormone / analysis
Microscopy, Electron
Microscopy, Immunoelectron
Middle Aged
Pituitary Neoplasms / chemistry,  pathology*,  ultrastructure
Tumor Cells, Cultured / chemistry,  pathology,  ultrastructure
Reg. No./Substance:
0/Chorionic Gonadotropin; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evaluation of osteonectin as a diagnostic marker of osteogenic bone tumors.
Next Document:  Flow cytometric DNA analysis of carcinoid tumors of the ileum and appendix.