Document Detail

Contribution of the endoplasmic reticulum to peroxisome formation.
MedLine Citation:
PMID:  16009135     Owner:  NLM     Status:  MEDLINE    
How peroxisomes are formed in eukaryotic cells is unknown but important for insight into a variety of diseases. Both human and yeast cells lacking peroxisomes due to mutations in PEX3 or PEX19 genes regenerate the organelles upon reintroduction of the corresponding wild-type version. To evaluate how and from where new peroxisomes are formed, we followed the trafficking route of newly made YFP-tagged Pex3 and Pex19 proteins by real-time fluorescence microscopy in Saccharomyces cerevisiae. Remarkably, Pex3 (an integral membrane protein) could first be observed in the endoplasmic reticulum (ER), where it concentrates in foci that then bud off in a Pex19-dependent manner and mature into fully functional peroxisomes. Pex19 (a farnesylated, mostly cytosolic protein) enriches first at the Pex3 foci on the ER and then on the maturing peroxisomes. This trafficking route of Pex3-YFP is the same in wild-type cells. These results demonstrate that peroxisomes are generated from domains in the ER.
Dominic Hoepfner; Danny Schildknegt; Ineke Braakman; Peter Philippsen; Henk F Tabak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  122     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-08-22     Revised Date:  2009-06-11    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Department of Cellular Protein Chemistry, University of Utrecht, Padualaan 8, NL-3548 CH Utrecht, The Netherlands.
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MeSH Terms
Bacterial Proteins / analysis,  genetics,  metabolism
Endoplasmic Reticulum / metabolism*,  ultrastructure
Luminescent Proteins / analysis,  genetics,  metabolism
Membrane Proteins / genetics,  metabolism
Microscopy, Fluorescence
Peroxisomes / metabolism*,  secretion*,  ultrastructure
Recombinant Fusion Proteins / analysis,  genetics,  metabolism
Saccharomyces cerevisiae / genetics,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Reg. No./Substance:
0/Bacterial Proteins; 0/Luminescent Proteins; 0/Membrane Proteins; 0/PEX19 protein, S cerevisiae; 0/PEX3 protein, S cerevisiae; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 0/yellow fluorescent protein, Bacteria
Comment In:
Curr Biol. 2005 Sep 20;15(18):R774-6   [PMID:  16169481 ]
Cell. 2005 Jul 15;122(1):1-2   [PMID:  16009123 ]

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