| Contribution of the endoplasmic reticulum to peroxisome formation. | |
| | |
MedLine Citation:
|
PMID: 16009135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
How peroxisomes are formed in eukaryotic cells is unknown but important for insight into a variety of diseases. Both human and yeast cells lacking peroxisomes due to mutations in PEX3 or PEX19 genes regenerate the organelles upon reintroduction of the corresponding wild-type version. To evaluate how and from where new peroxisomes are formed, we followed the trafficking route of newly made YFP-tagged Pex3 and Pex19 proteins by real-time fluorescence microscopy in Saccharomyces cerevisiae. Remarkably, Pex3 (an integral membrane protein) could first be observed in the endoplasmic reticulum (ER), where it concentrates in foci that then bud off in a Pex19-dependent manner and mature into fully functional peroxisomes. Pex19 (a farnesylated, mostly cytosolic protein) enriches first at the Pex3 foci on the ER and then on the maturing peroxisomes. This trafficking route of Pex3-YFP is the same in wild-type cells. These results demonstrate that peroxisomes are generated from domains in the ER. |
| | |
Authors:
|
Dominic Hoepfner; Danny Schildknegt; Ineke Braakman; Peter Philippsen; Henk F Tabak |
Related Documents
:
|
16803705 - Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (hne) o... 17376975 - Asymmetric distribution of prickle-like 2 reveals an early underlying polarization of v... 8662265 - The delayed rectifier, iki, is the major conductance in type i vestibular hair cells ac... 6195885 - Correlated tem, sem, and histological observations of filiform papillae of the cow tongue. 309625 - Non-specific acid esterase activity in rat lymphocytes. 12865435 - Caspase activation during phorbol ester-induced apoptosis requires rock-dependent myosi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell Volume: 122 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 2005 Jul |
Date Detail:
|
Created Date: 2005-07-12 Completed Date: 2005-08-22 Revised Date: 2009-06-11 |
Medline Journal Info:
|
Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 85-95 Citation Subset: IM |
Affiliation:
|
Department of Cellular Protein Chemistry, University of Utrecht, Padualaan 8, NL-3548 CH Utrecht, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins
/
analysis,
genetics,
metabolism Endoplasmic Reticulum / metabolism*, ultrastructure Luminescent Proteins / analysis, genetics, metabolism Membrane Proteins / genetics, metabolism Microscopy, Fluorescence Peroxisomes / metabolism*, secretion*, ultrastructure Recombinant Fusion Proteins / analysis, genetics, metabolism Saccharomyces cerevisiae / genetics, metabolism Saccharomyces cerevisiae Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Luminescent Proteins; 0/Membrane Proteins; 0/PEX19 protein, S cerevisiae; 0/PEX3 protein, S cerevisiae; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 0/yellow fluorescent protein, Bacteria |
| Comments/Corrections | |
Comment In:
|
Curr Biol. 2005 Sep 20;15(18):R774-6
[PMID:
16169481
]
Cell. 2005 Jul 15;122(1):1-2 [PMID: 16009123 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intracellular protein and DNA dynamics in competent Bacillus subtilis cells.
Next Document: Dynein anchors its mRNA cargo after apical transport in the Drosophila blastoderm embryo.