Document Detail


Contribution of L-type channels to Ca2+ regulation of neuronal properties in early developing purkinje neurons.
MedLine Citation:
PMID:  16035195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Activity driven Ca2+ signaling is an important regulator of neuronal development. Early developing Purkinje neurons (postnatal day 5-7) prior to the stage of dendritic development express a somatic Ca2+ signaling pathway that is electrically driven and communicates information from the cell membrane to the cytosol and nucleus. In the current studies, we examined the properties and potential functional role of this pathway using acutely isolated Purkinje neurons from postnatal day 5-7 rat pups and brief K+ stimulation to activate the pathway. Results show that the amplitude of the nuclear Ca2+ signal increases as a function of the cytosolic Ca2+ signal but is larger than the cytosolic Ca2+ signal at strong K+ stimulations. Both L-type and P-type Ca2+ channels contribute to the Ca2+ signal. We also show using semiquantitative immunohistochemical methods that activation of this Ca2+ signaling pathway results in activation the transcription factor CREB and that L-type Ca2+ channels play a prominent role in this effect. The level of cfos, a transcription factor whose expression is regulated by CREB, was also increased by K+ stimulation. K+ stimulation also altered the level of the Ca2+ binding protein calbindin, an effect that involved L-type Ca2+ channels. The relationship between increases in Ca2+ and calbindin expression was bell-shaped, with high levels of Ca2+ decreasing calbindin expression. The level of the transmitter GABA was also increased by K+ stimulation but this effect was not dependent on L-type Ca2+ channels. Taken together, these results support a role for L-type channels in the phenotypic expression of Purkinje neuron properties during early development and suggest that the different activity patterns of early developing Purkinje neurons could be one mechanism for signaling the induction of specific genes through differences in cytosolic or nuclear Ca2+.
Authors:
D L Gruol; J G Netzeband; L A Quina; P K Blakely-Gonzalez
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cerebellum (London, England)     Volume:  4     ISSN:  1473-4222     ISO Abbreviation:  Cerebellum     Publication Date:  2005  
Date Detail:
Created Date:  2005-07-22     Completed Date:  2005-08-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101089443     Medline TA:  Cerebellum     Country:  England    
Other Details:
Languages:  eng     Pagination:  128-39     Citation Subset:  IM    
Affiliation:
Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California, USA. gruol@scripps.edu
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Calcium / physiology*
Calcium Channel Blockers / pharmacology
Calcium Channels, L-Type / metabolism*
Cell Nucleus / drug effects,  metabolism
Cells, Cultured
Cerebellum / cytology*,  growth & development
Cyclic AMP Response Element-Binding Protein / metabolism
Cytosol / drug effects,  metabolism
Dose-Response Relationship, Drug
Drug Interactions
Electric Impedance
Electric Stimulation / methods
Extracellular Space / drug effects,  metabolism
Fura-2 / metabolism
Gene Expression Regulation / drug effects
Immunohistochemistry / methods
Membrane Potentials / drug effects,  physiology,  radiation effects
Methoxyhydroxyphenylglycol / analogs & derivatives,  pharmacology
Nimodipine / pharmacology
Patch-Clamp Techniques / methods
Potassium / pharmacology
Proto-Oncogene Proteins c-fos / metabolism
Purkinje Cells / drug effects,  metabolism*
Rats
Time Factors
gamma-Aminobutyric Acid / metabolism
omega-Conotoxins / pharmacology
Grant Support
ID/Acronym/Agency:
NS045339/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Calcium Channels, L-Type; 0/Cyclic AMP Response Element-Binding Protein; 0/Proto-Oncogene Proteins c-fos; 0/omega-Conotoxins; 147794-23-8/omega-conotoxin-MVIIC; 3343-19-9/dihydroxyphenylethylene glycol; 534-82-7/Methoxyhydroxyphenylglycol; 56-12-2/gamma-Aminobutyric Acid; 66085-59-4/Nimodipine; 7440-09-7/Potassium; 7440-70-2/Calcium; 96314-98-6/Fura-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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