| Contribution of IL-33 to induction and augmentation of experimental allergic conjunctivitis. | |
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MedLine Citation:
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PMID: 20501612 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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IL-33, a member of the IL-1 family of cytokines, is the ligand for ST2 (IL-33Ralpha chain). IL-33 has the capacity to induce T(h)2 cytokine production from T(h)2 cells, mast cells and basophils, indicating that IL-33 has the potential to induce T(h)2 cytokine-mediated allergic inflammation of the eye. Thus, we tested the pathological role of IL-33 in allergic conjunctivitis (AC). As reported elsewhere, animals immunized with ragweed pollen (RW)/alum and boosted with RW/PBS developed AC promptly (within 15 min) and conjunctival eosinophilic inflammation after a delay (within 24 h) in response to eye drop challenge with RW. Furthermore, RW-immunized mice, when topically challenged with both RW and IL-33, developed more striking eosinophilia in their conjunctiva without exacerbation of the clinical AC score. This in vivo IL-33 treatment significantly increased the capacity of T cells in the cervical lymph nodes of RW-immunized mice to produce IL-4, IL-5 and IL-13 upon challenge with anti-CD3 and anti-CD28 antibodies in vitro. Furthermore, the infiltrating cells were largely eosinophils and a small proportion of CD4(+) T cells, both of which express ST2. We also found that even splenic eosinophils express ST2 and show increased expression in response to IL-5, granulocyte-macrophage colony-stimulating factor (GM-CSF) or IL-33. Eosinophils, stimulated with IL-5 and/or GM-CSF, are responsive to IL-33, which induces production of IL-4 and chemokines. Finally, we showed that conjunctival tissues constitutively express biologically active IL-33, suggesting that IL-33 might play a crucial role in the induction and augmentation of AC. |
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Authors:
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Saori Matsuba-Kitamura; Tomohiro Yoshimoto; Koubun Yasuda; Shizue Futatsugi-Yumikura; Yuuko Taki; Taichiro Muto; Tomohiro Ikeda; Osamu Mimura; Kenji Nakanishi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-25 |
Journal Detail:
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Title: International immunology Volume: 22 ISSN: 1460-2377 ISO Abbreviation: Int. Immunol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-28 Completed Date: 2010-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8916182 Medline TA: Int Immunol Country: England |
Other Details:
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Languages: eng Pagination: 479-89 Citation Subset: IM |
Affiliation:
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Department of Immunology and Medical Zoology, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alum Compounds
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administration & dosage Ambrosia Animals Antigens, Plant / administration & dosage, immunology CD4-Positive T-Lymphocytes / drug effects*, immunology, metabolism, pathology Conjunctiva / drug effects*, immunology, metabolism, pathology Conjunctivitis, Allergic / immunology*, physiopathology Cytokines / biosynthesis, genetics, secretion Eosinophilia Humans Immunization, Secondary Interleukins / administration & dosage* Lymphocyte Activation / drug effects Mice Mice, Inbred BALB C Models, Animal Pollen / immunology Receptors, Interleukin / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Alum Compounds; 0/Antigens, Plant; 0/Cytokines; 0/Il1rl1 protein, mouse; 0/Interleukins; 0/Receptors, Interleukin; 0/interleukin-33, mouse; 10043-01-3/aluminum sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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