| Contribution of glycerol on production of poly(gamma-Glutamic Acid) in Bacillus subtilis NX-2. | |
| | |
MedLine Citation:
|
PMID: 18696262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Glycerol would stimulate the production of poly(gamma-glutamic acid) (gamma-PGA) and decrease its molecular weight in Bacillus subtilis NX-2. When 20 g/l glycerol was added in the medium, the yield of gamma-PGA increased from 26.7 +/- 1.0 to 31.7 +/- 1.3 g/l, and molecular weight of gamma-PGA decreased from 2.43 +/- 0.07 x 10(6) to 1.86 +/- 0.06 x 10(6) Da. In addition, it was found that the decrease of gamma-PGA chain length by glycerol would lead to the decrease of broth viscosity during the fermentation and enhanced the uptake of substrates, which could not only improve cell growth but also stimulate gamma-PGA production. Moreover, it was also found that glycerol could effectively regulate molecular weight between 2.43 +/- 0.07 x 10(6) and 1.42 +/- 0.05 x 10(6) Da with the concentration ranging from 0 to 60 g/l. This was the first time to discover such contribution of glycerol on gamma-PGA production in Bacillus genus. And the effects of glycerol on molecular weight of gamma-PGA would be developed to be an approach for the regulation of microbial gamma-PGA chain length, which is of practical importance for future commercial development of this polymer. |
| | |
Authors:
|
Qun Wu; Hong Xu; Jinfeng Liang; Jun Yao |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-12 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: 160 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-26 Completed Date: 2010-04-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 386-92 Citation Subset: IM |
Affiliation:
|
Nanjing University of Technology, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
chemistry,
growth & development,
metabolism* Culture Media / chemistry, metabolism Fermentation Glycerol / metabolism* Molecular Weight Polyglutamic Acid / analogs & derivatives*, chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid; 56-81-5/Glycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High Soluble Expression of D: -Amino Acid Oxidase in Escherichia coli Regulated by a Native Promoter...
Next Document: Activity of some Mucolytics Against Bacterial Adherence to Mammalian Cells.