| Contribution of Epstein-Barr virus infection to chemoresistance of gastric carcinoma cells to 5-fluorouracil. | |
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MedLine Citation:
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PMID: 21544729 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Although Epstein-Barr virus (EBV) is associated with 6-16% of the gastric carcinoma (GC) cases, the effect of EBV infection on the tumorigenesis process and the responsiveness to chemotherapy remain unclear. We compared chemosensitivity of the EBV-positive GC (AGSEBV) and EBV-negative GC (AGS) cells to 5-fluorouracil (5-FU). Although 5-FU inhibited the growth of both cell lines in a dose- and time-dependent manner, the sensitivity of EBV-positive GC cells to 5-FU was lower than that of EBV-negative GC cells. The cleavage of PARP and caspase-3 was also lower in AGS-EBV cells than in AGS cells following 5-FU treatment. Both the level of Bcl-2 expression and the ratio of Bcl-2/Bax were higher in AGS-EBV than in AGS cells not only at basal state but also following 5-FU treatment. Moreover, p53 and p21 expression was enhanced further by 5-FU in AGS than in AGS-EBV cells. Immunofluorescence assay and Western blot showed that 5-FU induced the expression of EBV-lytic genes including BZLF1, BRLF1, BMRF1 and BHRF1. Our results suggest that latent and lytic EBV infection contributes to the chemoresistance to 5-FU in gastric carcinoma by modulating apoptosis related cellular genes. |
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Authors:
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Jung Seon Seo; Tai-Gyu Kim; Young Seon Hong; Jen-Yang Chen; Suk Kyeong Lee |
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Publication Detail:
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Type: Journal Article Date: 2011-05-05 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 34 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 635-43 Citation Subset: IM |
Affiliation:
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Research Institute of Immunobiology, Department of Medical Lifescience, College of Medicine, Catholic University of Korea, Seoul, 137-701, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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