Document Detail


Contrast-enhanced ultrasound reveals real-time spatial changes in vascular perfusion during early implantation in the macaque uterus.
MedLine Citation:
PMID:  21316046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To use contrast-enhanced ultrasound (CEU) to quantify blood flow in the macaque uterus during early pregnancy.
DESIGN: Prospective nonhuman primate study.
SETTING: Oregon National Primate Research Center.
ANIMALS: Naturally cycling female rhesus macaques (Macaca mulatta).
INTERVENTION(S): Female macaques were mated on days 11-14 of the cycle. Females were then imaged by CEU and Doppler ultrasound once every 3 days from day 21 through day 39 of the fertile cycle.
MAIN OUTCOME MEASURE(S): Visualization and quantification of uterine vascular perfusion.
RESULT(S): CEU identified the primary placental disc and underlying vessels approximately 2 days earlier than Doppler ultrasound was able to observe endometrial thickening. CEU revealed spatial differences in vascular perfusion between the endometrium, myometrium, and endometrial-myometrial (junctional) zone. Myometrium displayed the highest rate of blood flow (>10 mL/min/g tissue). There was less blood flow in the endometrium and junctional zone (<3 mL/min/g). A brief fall in progesterone was observed during early implantation, which was correlated with reduced blood flow to all three uterine compartments, but did not reduce flow to the placenta.
CONCLUSIONS: CEU provides a sensitive, noninvasive method to assess vascular perfusion of the uterus during embryo implantation in macaques. We propose CEU as a new diagnostic tool to monitor vascular changes associated with early pregnancy in women.
Authors:
Christopher S Keator; Jonathan R Lindner; J Todd Belcik; Cecily V Bishop; Ov D Slayden
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Fertility and sterility     Volume:  95     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-05-12     Revised Date:  2012-04-02    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1316-21.e1-3     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006-3448, USA. keatorc@ohsu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Computer Systems*
Contrast Media / diagnostic use*
Embryo Implantation* / physiology
Female
Macaca mulatta
Pregnancy
Prospective Studies
Regional Blood Flow / physiology
Ultrasonography, Doppler
Ultrasonography, Prenatal* / methods
Uterus / blood supply*,  ultrasonography*
Grant Support
ID/Acronym/Agency:
NCRR 000163//PHS HHS; P51 RR000163-51/RR/NCRR NIH HHS; R01 DK063508-09/DK/NIDDK NIH HHS; R01 HL078610-08/HL/NHLBI NIH HHS; R01 HL078610-09/HL/NHLBI NIH HHS; R01-DK-063508/DK/NIDDK NIH HHS; R01-HL-078610/HL/NHLBI NIH HHS; RC1 HL100659-02/HL/NHLBI NIH HHS; RC1-HL-100659/HL/NHLBI NIH HHS; T32 HD007133-30/HD/NICHD NIH HHS; T32-HD007133/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media

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