Document Detail

Contrast-enhanced sonographic imaging of lymphatic channels and sentinel lymph nodes.
MedLine Citation:
PMID:  15972710     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The purpose of this study was to determine whether lymphatic channels (LCs) and sentinel lymph nodes (SLNs) could be detected on sonographic imaging after subcutaneous, submucosal, or parenchymal injections of a sonographic contrast agent (ie, lymphosonography) in a variety of anatomic locations in several animal models.
METHODS: Eight swine, 7 canines, 4 rabbits, and a monkey were used for these evaluations. Gray scale pulse inversion harmonic imaging of the LCs and the SLNs was performed after subcutaneous (n = 58), submucosal (n = 14), or parenchymal (n = 8) injections of a tissue-specific sonographic contrast agent (Sonazoid; GE Healthcare, Oslo, Norway). In many instances, blue dye was injected into the same locations as Sonazoid, and surgical dissection of the SLNs and LCs was performed for comparison. Scanning electron microscopy (SEM) of contrast-enhanced and control lymph nodes from 2 rabbits was performed to determine the mechanism of contrast agent uptake and retention within SLNs.
RESULTS: After subcutaneous, submucosal, or parenchymal contrast agent injections, gray scale pulse inversion harmonic imaging could be used to identify the number and location(s) of LCs and SLNs. After subcutaneous, submucosal, or parenchymal contrast agent injections, Sonazoid was confined to the SLNs (ie, contrast enhancement was not detected in the second-echelon nodes). There was good agreement between the results of lymphosonography and blue dye with surgical dissection in identifying the regional LCs and SLNs. Scanning electron microscopy identified vacuoles representing intact contrast microbubbles within contrast-enhanced SLN macrophages, which were not present in the control lymph nodes.
CONCLUSIONS: Lymphosonography can be used to detect lymphatic drainage pathways and SLNs in a variety of animal models.
Barry B Goldberg; Daniel A Merton; Ji-Bin Liu; George Murphy; Flemming Forsberg
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine     Volume:  24     ISSN:  0278-4297     ISO Abbreviation:  J Ultrasound Med     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-23     Completed Date:  2006-01-10     Revised Date:  2011-12-12    
Medline Journal Info:
Nlm Unique ID:  8211547     Medline TA:  J Ultrasound Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  953-65     Citation Subset:  IM    
The Jefferson Ultrasound Research and Education Institute, Department of Radiology, Thomas Jefferson University Hospital, Philadelphia, PA 19107, USA.
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MeSH Terms
Abdomen / ultrasonography
Contrast Media / administration & dosage*,  pharmacokinetics
Ferric Compounds / administration & dosage*,  pharmacokinetics
Gastrointestinal Tract / ultrasonography
Image Enhancement / methods*
Image Processing, Computer-Assisted / methods
Imaging, Three-Dimensional / methods
Injections / methods
Injections, Subcutaneous / methods
Iron / administration & dosage*,  pharmacokinetics
Lymph Node Excision / methods
Lymph Nodes / surgery,  ultrasonography*
Lymphatic Vessels / surgery,  ultrasonography*
Microscopy, Electron, Scanning / methods
Models, Animal
Oxides / administration & dosage*,  pharmacokinetics
Prostate / ultrasonography
Sentinel Lymph Node Biopsy / methods
Testis / ultrasonography
Ultrasonography, Doppler, Color / methods
Reg. No./Substance:
0/Contrast Media; 0/Ferric Compounds; 0/Oxides; 0/Sonazoid; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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