| Contrast enhanced maximum intensity projection ultrasound imaging for assessing angiogenesis in murine glioma and breast tumor models: A comparative study. | |
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MedLine Citation:
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PMID: 21144542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to prospectively compare noninvasive, quantitative measures of vascularity obtained from four contrast enhanced ultrasound (US) techniques to four invasive immunohistochemical markers of tumor angiogenesis in a large group of murine xenografts. Glioma (C6) or breast cancer (NMU) cells were implanted in 144 rats. The contrast agent Optison (GE Healthcare, Princeton, NJ) was injected in a tail vein (dose: 0.4ml/kg). Power Doppler imaging (PDI), pulse-subtraction harmonic imaging (PSHI), flash-echo imaging (FEI), and Microflow imaging (MFI; a technique creating maximum intensity projection images over time) was performed with an Aplio scanner (Toshiba America Medical Systems, Tustin, CA) and a 7.5MHz linear array. Fractional tumor neovascularity was calculated from digital clips of contrast US, while the relative area stained was calculated from specimens. Results were compared using a factorial, repeated measures ANOVA, linear regression and z-tests. The tortuous morphology of tumor neovessels was visualized better with MFI than with the other US modes. Cell line, implantation method and contrast US imaging technique were significant parameters in the ANOVA model (p<0.05). The strongest correlation determined by linear regression in the C6 model was between PSHI and percent area stained with CD31 (r=0.37, p<0.0001). In the NMU model the strongest correlation was between FEI and COX-2 (r=0.46, p<0.0001). There were no statistically significant differences between correlations obtained with the various US methods (p>0.05). In conclusion, the largest study of contrast US of murine xenografts to date has been conducted and quantitative contrast enhanced US measures of tumor neovascularity in glioma and breast cancer xenograft models appear to provide a noninvasive marker for angiogenesis; although the best method for monitoring angiogenesis was not conclusively established. |
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Authors:
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Flemming Forsberg; Raymond J Ro; Traci B Fox; Ji-Bin Liu; See-Ying Chiou; Magdalena Potoczek; Barry B Goldberg |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-18 |
Journal Detail:
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Title: Ultrasonics Volume: 51 ISSN: 1874-9968 ISO Abbreviation: Ultrasonics Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-01-07 Completed Date: 2011-02-17 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 0050452 Medline TA: Ultrasonics Country: Netherlands |
Other Details:
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Languages: eng Pagination: 382-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Radiology, Thomas Jefferson University, Philadelphia, PA 19107, USA. flemming.forsberg@jefferson.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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administration & dosage* Analysis of Variance Animals Cell Line, Tumor Contrast Media / administration & dosage* Female Fluorocarbons / administration & dosage* Glioma / blood supply*, ultrasonography* Immunohistochemistry Linear Models Mammary Neoplasms, Experimental / blood supply*, ultrasonography* Neovascularization, Pathologic / ultrasonography* Prospective Studies Rats Rats, Sprague-Dawley Ultrasonography / methods* |
| Grant Support | |
ID/Acronym/Agency:
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CA093907/CA/NCI NIH HHS; R21 CA093907-01A2/CA/NCI NIH HHS; R21 CA093907-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Contrast Media; 0/FS 069; 0/Fluorocarbons |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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