| Contrast-enhanced CT of Articular Cartilage: Experimental Study for Quantification of Glycosaminoglycan Content in Articular Cartilage. | |
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MedLine Citation:
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PMID: 21940505 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose:To investigate the diagnostic potential of delayed contrast material-enhanced computed tomography (CT) of articular cartilage in quantification of glycosaminoglycan (GAG) concentration in normal and degenerated articular cartilage ex vivo by using a clinical CT scanner.Materials and Methods:This study was exempted by the institutional and animal review boards, and informed consent was not required. Forty intact porcine patellae were extracted and assigned to either a control (n = 20) or a trypsin-treated group (ie, GAG-depleted group) (n = 20). Ten patellae in each group were immersed in anionic contrast agent (ioxaglate, 40%) and the other ten in neutral contrast agent (iopromide, 35%) for 2 hours. To determine the contrast agent concentration within cartilage, samples were scanned with a clinical CT scanner immediately after the immersion time, and the x-ray attenuation of cartilage was measured. CT images were compared with safranin O-stained histologic sections, and actual GAG contents were determined with a dimethylmethylene blue assay.Results:Ioxaglate was taken up by GAG-depleted cartilage to a greater extent than by normal cartilage (P = .01). In contrast, the penetration of iopromide was not significantly different between GAG-depleted and normal cartilage (P = .1). The loss of GAGs in trypsin-treated cartilage was confirmed microscopically by using safranin O-stained sections, and a dimethylmethylene blue assay also confirmed that GAG content was markedly decreased in trypsin-treated cartilage (P = .003).Conclusion:This study showed that contrast-enhanced CT images of articular cartilage could reflect the GAG content within the cartilage by allowing measurement of the concentration of anionic iodine-based contrast agent accumulated in the cartilage.© RSNA, 2011. |
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Authors:
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Hye Jin Yoo; Sung Hwan Hong; Ja-Young Choi; In Joon Lee; Su Jin Kim; Jung-Ah Choi; Heung Sik Kang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-21 |
Journal Detail:
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Title: Radiology Volume: - ISSN: 1527-1315 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401260 Medline TA: Radiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Radiology and Institute of Radiation Medicine, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehang-Ro, Jongno-Gu, Seoul 110-744, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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