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Contrast agent-free sonoporation: The use of an ultrasonic standing wave microfluidic system for the delivery of pharmaceutical agents.
MedLine Citation:
PMID:  22662060     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Sonoporation is a useful biophysical mechanism for facilitating the transmembrane delivery of therapeutic agents from the extracellular to the intracellular milieu. Conventionally, sonoporation is carried out in the presence of ultrasound contrast agents, which are known to greatly enhance transient poration of biological cell membranes. However, in vivo contrast agents have been observed to induce capillary rupture and haemorrhage due to endothelial cell damage and to greatly increase the potential for cell lysis in vitro. Here, we demonstrate sonoporation of cardiac myoblasts in the absence of contrast agent (CA-free sonoporation) using a low-cost ultrasound-microfluidic device. Within this device an ultrasonic standing wave was generated, allowing control over the position of the cells and the strength of the acoustic radiation forces. Real-time single-cell analysis and retrospective post-sonication analysis of insonated cardiac myoblasts showed that CA-free sonoporation induced transmembrane transfer of fluorescent probes (CMFDA and FITC-dextran) and that different mechanisms potentially contribute to membrane poration in the presence of an ultrasonic wave. Additionally, to the best of our knowledge, we have shown for the first time that sonoporation induces increased cell cytotoxicity as a consequence of CA-free ultrasound-facilitated uptake of pharmaceutical agents (doxorubicin, luteolin, and apigenin). The US-microfluidic device designed here provides an in vitro alternative to expensive and controversial in vivo models used for early stage drug discovery, and drug delivery programs and toxicity measurements.
Authors:
Dario Carugo; Dyan N Ankrett; Peter Glynne-Jones; Lorenzo Capretto; Rosemary J Boltryk; Xunli Zhang; Paul A Townsend; Martyn Hill
Publication Detail:
Type:  Journal Article     Date:  2011-11-15
Journal Detail:
Title:  Biomicrofluidics     Volume:  5     ISSN:  1932-1058     ISO Abbreviation:  Biomicrofluidics     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-06-04     Completed Date:  2012-08-23     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  101293825     Medline TA:  Biomicrofluidics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44108-4410815     Citation Subset:  -    
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