Document Detail


Contracture of guinea-pig ileum on withdrawal of methionine5-enkephalin is mediated by substance P.
MedLine Citation:
PMID:  6085949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of methionine5-enkephalin (Met-enkephalin, ME) 5 X 10(-8)-5 X 10(-6) moll-1) were investigated on the resting guinea-pig ileum. While in contact with the ileum, ME reduced the natural tone and movements, but following washout a contracture occurred which increased with increasing duration of the contact period from 0.5 to 32 min and with increasing concentration of the ME present during the contact period. The washout contractures after 2 min contact with ME, 10(-6) moll-1, were abolished by naloxone, 10(-6)moll-1, added prior to the addition of ME, atropine, 5 X 10(-6)moll-1 and the substance P (SP) antagonist, (D-Pro2, D-Phe7, D-Trp9)-SP, 10(-5)moll-1 and were reduced by 5-hydroxytryptamine (5-HT)-autodesensitization. Washout contractures following 32 min contact with ME, 10(-6)moll-1, were significantly inhibited by the SP antagonist and naloxone and were abolished by a combination of atropine and the SP antagonist, but were not significantly reduced by atropine alone or by 5-HT-desensitization. Contractures of ileum occurred on addition of naloxone to ileal segments exposed to ME for 2 or 32 min. These contractures were also inhibited by the SP antagonist but a combination of atropine and SP-desensitization was required to abolish them. It was concluded that gut dependence occurs following very brief exposure to an opioid and that SP plays a central role in the withdrawal response precipitated either by washout or addition of naloxone.
Authors:
L A Chahl
Related Documents :
6125329 - Oxygen consumption of the dogfish gill tissue following zinc treatment.
12600759 - Fluctuations of exposure rate at regional radiation monitoring stations in korea.
15633599 - Annoyance and performance of three environmentally intolerant groups during experimenta...
24149699 - The effects of regular aerobic exercise on renal functions in streptozotocin induced di...
9759809 - Levels of carbon dioxide in helmet systems used during orthopaedic operations.
10472319 - Compromised concentrations of ascorbate in fluid lining the respiratory tract in human ...
3246259 - St segment changes in healthy volunteers during holter monitoring and exercise stress t...
1459749 - Physiological changes in male competitive cyclists after two weeks of intensified train...
11760269 - Exercise behavior among female occupational health nurses. influence of self efficacy, ...
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  80     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1987-01-21     Completed Date:  1987-01-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  741-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Enkephalin, Methionine / pharmacology*
Feedback
Guinea Pigs
Muscle Contraction / drug effects*
Muscle, Smooth / drug effects*
Naloxone / pharmacology
Substance P / physiology*
Substance Withdrawal Syndrome / chemically induced,  physiopathology*
Chemical
Reg. No./Substance:
33507-63-0/Substance P; 465-65-6/Naloxone; 58569-55-4/Enkephalin, Methionine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adenosine inhibits and potentiates IgE-dependent histamine release from human basophils by an A2-rec...
Next Document:  Serum dopamine-beta-hydroxylase activity: clinical applications in child psychiatry.