| Contraction-mediated glycogenolysis in mouse skeletal muscle lacking creatine kinase: the role of phosphorylase b activation. | |
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MedLine Citation:
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PMID: 12963789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Skeletal muscle that is deficient in creatine kinase (CK-/-) exhibits accelerated glycogenolysis during contraction. Understanding this phenomenon could provide insight into the control of glycogenolysis during contraction. Therefore, glycogen breakdown was investigated in isolated extensor digitorum longus CK-/- muscle. Muscles were stimulated to produce repeated tetani for 20 s in the presence of sodium cyanide to block mitochondrial respiration. Accumulation of lactate after stimulation was similar in wild-type (WT) and CK-/- muscles, whereas accumulation of glucose-6-phosphate was twofold higher in CK-/- muscles, indicating greater glycogenolysis in CK-/- muscles. Total phosphorylase activity was decreased by almost 30 % in CK-/- muscle (P < 0.001). Phosphorylase fractional activity (-/+ 3.3 mM AMP) was similar in both groups in the basal state (about 10 %), but increased to a smaller extent in CK-/- muscles after stimulation (39 +/- 4 % vs. 52 +/- 4 % in WT, P < 0.05). Inorganic phosphate, the substrate for phosphorylase, increased marginally in CK-/- muscles after stimulation (basal = 25.3 +/- 2.2 micromol (g dry muscle)-1; stimulated = 33.9 +/- 2.3 micromol (g dry muscle)-1), but substantially in WT muscles (basal = 11.4 +/- 0.7 micromol (g dry muscle)-1; stimulated = 54.2 +/- 4.5 micromol (g dry muscle)-1). Kinetic studies of phosphorylase b (dephosphorylated enzyme) from muscle extracts in vitro demonstrated higher relative activities in CK-/- muscles (60-135 %) in response to low AMP concentrations (up to 50 microM) in both the basal state and after stimulation (P < 0.05), whereas no differences in activity between CK-/- and WT muscles were observed at high AMP concentrations (> 100 microM). These data indicate that allosteric activation of phosphorylase b accounts for the accelerated glycogenolysis in CK-/- muscle during contraction. |
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Authors:
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Abram Katz; Daniel C Andersson; Josephine Yu; Barbara Norman; Marie E Sandstrom; Be Wieringa; Hakan Westerblad |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2003-09-08 |
Journal Detail:
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Title: The Journal of physiology Volume: 553 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-03 Completed Date: 2004-09-23 Revised Date: 2013-06-09 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 523-31 Citation Subset: IM |
Affiliation:
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Department of Physiology and Pharmacology, Karolinska Institutet, 17177 Stockholm, Sweden. abram.katz@fyfa.ki.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Monophosphate / metabolism, pharmacology Adenosine Triphosphate / metabolism Animals Creatine / metabolism Creatine Kinase / genetics* Electric Stimulation Enzyme Activation / drug effects Glucose-6-Phosphate / metabolism Glycogen / metabolism* Glycogen Phosphorylase / metabolism Glycogen Synthase / metabolism Inosine / metabolism Inosine Monophosphate / metabolism Lactic Acid / metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Muscle Contraction / physiology* Muscle Fatigue / physiology Muscle, Skeletal / metabolism* Phosphocreatine / metabolism Phosphorylase a / metabolism Phosphorylase b / metabolism* Sodium Cyanide / pharmacology |
| Chemical | |
Reg. No./Substance:
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131-99-7/Inosine Monophosphate; 143-33-9/Sodium Cyanide; 50-21-5/Lactic Acid; 56-65-5/Adenosine Triphosphate; 56-73-5/Glucose-6-Phosphate; 57-00-1/Creatine; 58-63-9/Inosine; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 67-07-2/Phosphocreatine; 9005-79-2/Glycogen; EC 2.4.1.-/Glycogen Phosphorylase; EC 2.4.1.-/Phosphorylase a; EC 2.4.1.-/Phosphorylase b; EC 2.4.1.11/Glycogen Synthase; EC 2.7.3.2/Creatine Kinase |
| Comments/Corrections | |
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