Document Detail


Contractility studies on isolated bovine choroidal small arteries: determination of the active and passive wall tension-internal circumference relation.
MedLine Citation:
PMID:  12384087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies on isolated choroidal arteries could help to understand the regulatory mechanisms in the choroidal circulation. The aim of the present study was therefore to assess whether contractility studies on isolated choroidal arteries were feasible and to determine the active and passive wall tension-internal circumference relation of these arteries. This relation is essential for reliable further pharmacodynamic studies on these vessels. Isolated choroidal arteries were mounted on a wire myograph for isometric tension recording. After the vessel was mounted, the L(100) (the circumference of the vessel at a transmural pressure of 100 mmHg) was determined. Then the passive and active wall tension-internal circumference relation of the choroidal vessels was obtained by stepwise increasing the internal circumference. The changes in the internal circumference were expressed as a percentage of L(100). After each increase in circumference, the passive tone (in a calcium free medium), the spontaneous tone (in a Krebs--Ringer bicarbonate solution) and the active tone (in a solution containing K(+) 120 mM and prostaglandin F(2 alpha) 30 microM) was measured. The passive tone of the vessel increased exponentially with the circumference of the vessel. Both the spontaneous tone and the active tone also increased when the vessel was stretched. They peaked when the internal circumference approached 90% of the L(100) and diminished again when the circumference was further increased. The peak value of the active tension curve averaged 2.24+/-0.47 Nm(-1) (n=10). The passive tension was 0.57+/-0.08 Nm(-1) (n=10) at this circumference. The peak value of the spontaneous tension curve averaged 0.37+/-0.08 Nm(-1) (n=10). It can be concluded that in vitro contractility studies on isolated choroidal arteries are feasible. The optimal length or preload of the choroidal arteries is attained when the internal circumference of the artery is set to 90% of the L(100).
Authors:
C Delaey; K Boussery; J Van de Voorde
Related Documents :
21555357 - Notice of concern.
14581717 - Effect of the vascular endothelium on contractions induced by noradrenaline and phenyle...
12181117 - Effect of lower limb exercise on forearm vascular function: contribution of nitric oxide.
22302377 - The effect of acute exercise with increasing workloads on inactive muscle blood flow an...
24680647 - The immunomodulating role of exercise in metabolic disease.
3198547 - The effects of nighttime seat belt enforcement on seat belt use by tavern patrons: a pr...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental eye research     Volume:  75     ISSN:  0014-4835     ISO Abbreviation:  Exp. Eye Res.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-10-17     Completed Date:  2002-11-06     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0370707     Medline TA:  Exp Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Affiliation:
Department of Physiology and Physiopathology, Ghent University, De Pintelan 185, Blok B, B-9000, Ghent, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / physiology
Biomechanics
Cattle
Choroid / blood supply*
Feasibility Studies
Stress, Mechanical
Vascular Resistance / physiology
Vasoconstriction

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of the peptide mycotoxin destruxin E on insect haemocytes and on dynamics and efficiency of ...
Next Document:  Expression patterns of cytochrome P4501B1 (Cyp1b1) in FVB/N mouse eyes.