Document Detail

Contractile ring-independent localization of DdINCENP, a protein important for spindle stability and cytokinesis.
MedLine Citation:
PMID:  16339076     Owner:  NLM     Status:  MEDLINE    
Dictyostelium DdINCENP is a chromosomal passenger protein associated with centromeres, the spindle midzone, and poles during mitosis and the cleavage furrow during cytokinesis. Disruption of the single DdINCENP gene revealed important roles for this protein in mitosis and cytokinesis. DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule-depolymerizing drugs, suggesting that their spindles may not be stable. Furthermore DdCP224, a protein homologous to the microtubule-stabilizing protein TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells. Overexpression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells. Although not required for the localization of the myosin II contractile ring and subsequent formation of a cleavage furrow, DdINCENP is important for the abscission of daughter cells at the end of cytokinesis. Finally, we show that the localization of DdINCENP at the cleavage furrow is modulated by myosin II but it occurs by a mechanism different from that controlling the formation of the contractile ring.
Qian Chen; Hui Li; Arturo De Lozanne
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-12-07
Journal Detail:
Title:  Molecular biology of the cell     Volume:  17     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-25     Completed Date:  2006-04-07     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  779-88     Citation Subset:  IM    
Institute of Cellular and Molecular Biology and Department of Molecular, Cellular, and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA.
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MeSH Terms
Cell Line
Chromosomal Proteins, Non-Histone / analysis,  genetics,  physiology*
Cloning, Molecular
Cytokinesis / physiology
Dictyostelium / cytology,  genetics,  metabolism*
Microtubule-Associated Proteins / analysis
Mitosis / genetics,  physiology
Mitotic Spindle Apparatus / chemistry,  metabolism*
Myosin Type II / metabolism
Protozoan Proteins / analysis,  genetics,  physiology*
Recombinant Fusion Proteins / analysis
Sequence Homology, Amino Acid
Grant Support
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/Microtubule-Associated Proteins; 0/Protozoan Proteins; 0/Recombinant Fusion Proteins; EC 3.6.1.-/Myosin Type II

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