| Contractile activity of lymphatic vessels is altered in the TNBS model of guinea pig ileitis. | |
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MedLine Citation:
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PMID: 16675748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of the lymphatic system to actively remove fluid from the interstitium is critical to the resolution of edema. The response of the lymphatics to inflammatory situations is poorly studied, so we examined mesenteric lymphatic contractile activity in the 2,4,6-trinitrobenzenesulfonic acid (TNBS) model of guinea pig ileitis, a well-accepted animal model of intestinal inflammation, by videomicroscopy in vivo and in vitro 1, 3, and 6 days after induction of ileitis. Lymphatic function (diameter, constriction frequency, amplitude of constrictions, and calculated stroke volume and lymph flow rate) of isolated vessels from TNBS-treated guinea pigs were impaired compared with sham-treated controls. The dysfunction was well correlated with the degree of inflammation, with differences reaching significance (P < 0.05) at the highest inflammation-induced damage observed at day 3. In vivo, significantly fewer lymphatics exhibited spontaneous constrictions in TNBS-treated than sham-treated animals. Cyclooxygenase (COX) metabolites were suggested to be involved in this lymphatic dysfunction, since application of nonselective COX inhibitor (10 microM indomethacin) or a combination of COX-1 and COX-2 inhibitors (1 microM SC-560 and 10 microM celecoxib) markedly increased constriction frequency or induced them in lymphatics from TNBS-treated animals in vivo and in vitro. The present results demonstrate that lymphatic contractile function is altered in TNBS-induced ileitis and suggest a role for prostanoids in the lymphatic dysfunction. |
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Authors:
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Theresa F Wu; Colin J Carati; Wallace K Macnaughton; Pierre-Yves von der Weid |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-04 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 291 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-08 Completed Date: 2006-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G566-74 Citation Subset: IM |
Affiliation:
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Dept. of Physiology and Biophysics, Univ. of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Guinea Pigs Ileitis / chemically induced*, physiopathology* Lymphatic Vessels / drug effects*, physiopathology* Male Muscle Contraction / drug effects, physiology Muscle, Smooth / drug effects, physiopathology* Prostaglandin-Endoperoxide Synthases / metabolism Trinitrobenzenesulfonic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
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2508-19-2/Trinitrobenzenesulfonic Acid; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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