Document Detail


Continuously generated H2O2 stimulates the proliferation and osteoblastic differentiation of human periodontal ligament fibroblasts.
MedLine Citation:
PMID:  22173791     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous studies have shown that hydrogen peroxide (H(2)O(2)) inhibits proliferation and osteoblastic differentiation in bone-like cells. Human periodontal ligament fibroblasts (PLF) are capable of differentiating into osteoblasts and are exposed to oxidative stress during periodontal inflammation. However, the cellular responses of PLF to H(2)O(2) have not been identified. In this study, we examined how H(2)O(2) affects the viability and proliferation of PLF by exposing the cells to glucose oxidase (GO) or direct addition of H(2)O(2). We also explored the effects of GO on the osteoblastic differentiation of PLF and the mechanisms involved. The viability and proliferation in PLF were increased with the addition of 10 mU/ml GO but not by volumes greater than 15 mU/ml or by H(2)O(2) itself. GO-stimulated DNA synthesis was correlated with the increase in cyclin E protein levels in the cells. Osteoblastic differentiation of PLF was also augmented by combined treatment with GO, as evidenced by the increases in alkaline phosphatase activity, mineralization, collagen synthesis, and osteocalcin content in the cells. The inductions of runt-related transcription factor 2 and osterix mRNA and proteins were further increased in PLF incubated in combination with GO compared to those in untreated cells. These results demonstrate that the continuous presence of H(2)O(2) stimulates the proliferation of PLF and augments their potential to differentiate into osteoblasts through the up-regulation of bone-specific transcription factors. Collectively, we suggest that H(2)O(2) may elicit the functions of PLF in maintaining the dimensions of the periodontal ligament and in mediating a balanced metabolism in alveolar bone.
Authors:
Youngji Choe; Ji-Yeon Yu; Young-Ok Son; Seung-Moon Park; Jong-Ghee Kim; Xianglin Shi; Jeong-Chae Lee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  113     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-05-31     Completed Date:  2012-10-11     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1426-36     Citation Subset:  IM    
Copyright Information:
© 2011 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Blotting, Western
Cell Differentiation / drug effects*
Cell Proliferation / drug effects*
Collagen / metabolism
Culture Media
DNA Primers
Fibroblasts / cytology,  drug effects
Humans
Hydrogen Peroxide / pharmacology*
Male
Osteoblasts / cytology,  drug effects*,  metabolism
Osteocalcin / metabolism
Periodontal Ligament / cytology,  drug effects*,  metabolism
Real-Time Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
R01 CA116697/CA/NCI NIH HHS; R01 CA116697-05/CA/NCI NIH HHS; R01 ES015518/ES/NIEHS NIH HHS; R01 ES015518-05/ES/NIEHS NIH HHS; R01CA116697/CA/NCI NIH HHS; R01ES015518/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA Primers; 104982-03-8/Osteocalcin; 9007-34-5/Collagen; BBX060AN9V/Hydrogen Peroxide
Comments/Corrections

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