Document Detail


Continuous uroflow cystometry in the urethane-anesthetized mouse.
MedLine Citation:
PMID:  20127833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: In vivo animal cystometry represents an accepted methodology for the study of lower urinary tract physiology. A particular advantage of the mouse model is the availability of genetically modified strains, offering the possibility of linking individual genes to relevant physiological events. However, small voided volumes complicate the ability to obtain reliable pressure-flow data by gravimetric methods, due to non-continuous drop formation and release during voiding. We investigated the feasibility of a simple non-gravimetric continuous urine collection system during cystometry under urethane anesthesia, and compared urethane-anesthetized with awake cystometry.
METHODS: Cystometry was performed in awake and urethane-anesthetized female mice using a suprapubic tube. A simple, novel non-gravimetric method of urine collection was used in urethane-anesthetized animals to assess voided volume and permit flow rate calculations. Pressure and time-related variables were compared between groups.
RESULTS: Voided urine collection appears to be complete and continuous in this model. Mean voided volume was 0.09 ± 0.020 ml, with an average flow rate of 0.029 ± 0.007 ml/sec. Urethane anesthesia delayed cystometric pressure/volume responses. However, micturition reflexes were intact and otherwise comparable between groups. Female mice void with pulsatile pressurization previously described in rats.
CONCLUSION: Suprapubic voiding cystometry using a simple and reliable urine collection method under urethane anesthesia is feasible in mice, permitting the integration of voided volumes with pressure and time data. The inclusion of volume and flow data enhances the usefulness of the mouse model for in vivo assessment of detrusor and potentially sphincteric performance.
Authors:
Phillip P Smith; George A Kuchel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurourology and urodynamics     Volume:  29     ISSN:  1520-6777     ISO Abbreviation:  Neurourol. Urodyn.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-01-27     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8303326     Medline TA:  Neurourol Urodyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1344-9     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Affiliation:
Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA. ppsmith@uchc.edu
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MeSH Terms
Descriptor/Qualifier:
Anesthesia, General*
Anesthetics, Intravenous*
Animals
Consciousness
Feasibility Studies
Female
Mice
Mice, Inbred C57BL
Models, Animal
Pressure
Reproducibility of Results
Time Factors
Transducers, Pressure
Urethane*
Urinary Bladder / physiology*
Urinary Catheterization* / instrumentation
Urination
Urodynamics*
Grant Support
ID/Acronym/Agency:
5R01AG028657/AG/NIA NIH HHS; R01 AG028657-01A1/AG/NIA NIH HHS; R01 AG028657-02/AG/NIA NIH HHS; R01 AG028657-03/AG/NIA NIH HHS; R01 AG028657-03S1/AG/NIA NIH HHS; R01 AG028657-03S2/AG/NIA NIH HHS; R01 AG028657-04/AG/NIA NIH HHS; R01 AG028657-05/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Anesthetics, Intravenous; 51-79-6/Urethane
Comments/Corrections

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