Document Detail

Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients.
MedLine Citation:
PMID:  15914850     Owner:  NLM     Status:  MEDLINE    
In order to investigate hepatitis C virus (HCV) persistence and replication in peripheral blood mononuclear cells (PBMC) from a group of haemophilic individuals, HCV production and release to PBMC culture supernatants (SNs) from HCV singly infected patients and HIV/HCV co-infected patients was studied. HCV RNA+ SNs were found more frequently from HIV/HCV co-infected individuals (89.5 %) with poor reconstitution of their immune status than from singly HCV-infected patients (57 %) or from HIV/HCV co-infected individuals with a good response to highly active anti-retroviral therapy (50 %). The presence of the HCV genome in culture SNs was associated with lower CD4+ T-cell counts and with a more severe clinical picture of HIV infection. In spite of prolonged negative HCV viraemia, PBMC from HIV/HCV co-infected patients released the HCV genome after culture. HCV permissive PBMC allowed generation of HCV productive B cell lines with continuous HCV replication. These findings add further weight to the involvement of PBMCs in persistence of HCV infection and emphasize the role of B lymphocytes as HCV reservoirs.
Patricia Baré; Ivana Massud; Cecilia Parodi; Liliana Belmonte; Gabriel García; Marcelo Campos Nebel; Marcelo Corti; Miguel Tezanos Pinto; Raúl Pérez Bianco; María M Bracco; Rodolfo Campos; Beatriz Ruibal Ares
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  86     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-25     Completed Date:  2005-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1717-27     Citation Subset:  IM    
Instituto de Investigaciones Hematológicas Mariano R Castex, Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires 1425, Argentina.
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MeSH Terms
Anti-HIV Agents / therapeutic use
Antiretroviral Therapy, Highly Active
B-Lymphocytes / virology
CD4 Lymphocyte Count
Cell Line, Tumor / virology
Cells, Cultured
HIV Infections / complications,  drug therapy
Hepacivirus / genetics,  isolation & purification*
Hepatitis C, Chronic / complications,  immunology,  virology*
Leukocytes, Mononuclear / virology*
RNA, Viral / analysis
Time Factors
Reg. No./Substance:
0/Anti-HIV Agents; 0/RNA, Viral

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