Document Detail


Continuous production of oxytetracycline in fluidized bed bioreactor by immobilized cells of Streptomyces varsoviensis MTCC-1537: effect of dilution and glucose loading rates.
MedLine Citation:
PMID:  19421724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxytetracycline (OT) production using glutaraldehyde cross-linked calcium alginate immobilized cells of Streptomyces varsoviensis in continuous fluidized bed reactor (FBR) was investigated. Initially, batch experiments were carried in stirred tank reactor (STR) and FBR using calcium alginate immobilized cells. Higher OT production of 0.45 gm/L was achieved by FBR when compared with 0.33 g/L of OT in STR. All subsequent studies were carried out in continuous mode of operation in FBR. During 21 days of operation, effect of glucose concentration and different dilution rates were studied. A maximum of 0.75 g/L OT was achieved in the medium having 10 g/L of glucose concentration. The highest OT concentration of 0.92 g/L and the highest yield of OT with respect to biomass at 0.1713 g/g were obtained at the dilution rate of 0.25 day(-1).
Authors:
Annapurna Jetty; Ravichandra Potumarthi; A Gangagni Rao; B Sarva Rao; S V Ramakrishna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-07
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  159     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2009-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  579-88     Citation Subset:  IM    
Affiliation:
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology (CSIR), Tarnaka, Hyderabad, 50000 India. annapurna@iict.res.in
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MeSH Terms
Descriptor/Qualifier:
Bioreactors / microbiology*
Cell Culture Techniques / methods*
Computer Simulation
Glucose / chemistry,  metabolism*
Models, Biological*
Oxytetracycline / isolation & purification,  metabolism*
Solubility
Species Specificity
Streptomyces / classification,  metabolism*
Chemical
Reg. No./Substance:
50-99-7/Glucose; 79-57-2/Oxytetracycline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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