Document Detail


Continuous perivascular L-arginine delivery increases total vessel area and reduces neointimal thickening after experimental balloon dilatation.
MedLine Citation:
PMID:  10073985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to evaluate whether vascular remodeling and neointimal thickening occur after balloon dilatation of the nonatherosclerotic rabbit carotid artery, and whether both processes are influenced by continuous perivascular delivery of L-arginine or the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). In the first experiment, histological and morphometric evaluation of arteries was performed at different time points after balloon dilatation: 10 minutes (n=7), and 1 (n=7), 2 (n=9), 3 (n=20), or 10 (n=5) weeks. Neointimal thickening progressively contributed to luminal narrowing for at least 10 weeks after angioplasty. During the first 2 weeks after dilatation, a significant decrease of the total vessel area was measured. Ten weeks after dilatation, both the neointimal and total vessel area were increased without further changing of the luminal area. In the second experiment, endothelial injured rabbits were randomly assigned to receive 2 weeks of continuous local perivascular physiological salt solution (n=6), L-arginine (n=8), or L-NAME (n=7), starting immediately after balloon dilatation (ie, local drug delivery during the first phase of the biphasic vascular remodeling process). Perivascular L-arginine delivery significantly reduced the neointimal area, despite an increased number of neointimal Ki-67-positive smooth muscle cells. Both the luminal area and total vessel area were significantly increased. Serum L-arginine levels remained unchanged. L-NAME administration had no effect on the neointimal area, nor on the luminal and total vessel area. Neointimal formation and biphasic vascular remodeling occur after experimental balloon dilatation of the nonatherosclerotic rabbit carotid artery, and can be influenced by continuous local perivascular delivery of L-arginine.
Authors:
J M Bosmans; C J Vrints; M M Kockx; H Bult; K M Cromheeke; A G Herman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  19     ISSN:  1079-5642     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-02     Completed Date:  1999-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  767-76     Citation Subset:  IM    
Affiliation:
Department of Cardiology and Pharmacology, University of Antwerp (UIA), Wilrijk, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arginine / pharmacology*
Balloon Dilatation / adverse effects*
Carotid Arteries / metabolism,  pathology
Carotid Artery Injuries*
Carotid Stenosis / metabolism,  pathology
Enzyme Inhibitors / pharmacology
Ki-67 Antigen / analysis
Male
Muscle, Smooth, Vascular / chemistry,  drug effects*,  metabolism*
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / metabolism
Rabbits
Time Factors
Tunica Intima / drug effects,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Ki-67 Antigen; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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