Document Detail


Continuous perifusion of dispersed anterior pituitary cells: technical aspects.
MedLine Citation:
PMID:  6669039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Continuous perifusion of dispersed anterior pituitary cells is a powerful dynamic in vitro technique that complements the static incubation technique of primary culture. The major advantages of perifusion include the ability to challenge cells with test substance in a pulsatile manner and to monitor the immediate response. Furthermore, the accumulation of hormonal product and proteolytic enzymes, with their potential effects on cell function, is essentially eliminated as a concern. Finally, although methods using static techniques are available, experiments using continuously perifused dispersed cells can be initiated soon after removal of the gland from the donor animal. We and others have found this system to be useful in the study of hormone secretion by the anterior pituitary. We expect that the system will lend itself equally well to investigations of other cellular processes involving the export of organic and inorganic substances.
Authors:
W S Evans; M J Cronin; M O Thorner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Methods in enzymology     Volume:  103     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  1983  
Date Detail:
Created Date:  1984-04-26     Completed Date:  1984-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  294-305     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods
Cells, Cultured
Culture Techniques / methods
Indicators and Reagents
Perfusion / instrumentation,  methods
Pituitary Gland, Anterior / cytology*,  metabolism
Grant Support
ID/Acronym/Agency:
1K04NS500601/NS/NINDS NIH HHS; HD00439/HD/NICHD NIH HHS; NS18409/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Indicators and Reagents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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