| Continuous perfusion of donor hearts in the beating state extends preservation time and improves recovery of function. | |
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MedLine Citation:
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PMID: 9806389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Improving methods of donor heart preservation may permit prolonged storage and remote procurement of cardiac allografts. We hypothesized that continuous, sanguineous perfusion of the donor heart in the beating, working state may prolong myocardial preservation. METHODS: We developed a portable perfusion apparatus for use in donor heart preservation. Contractile, metabolic, and vasomotor functions were monitored simultaneously in an isolated swine heart. The metabolic state was monitored by myocardial tissue pH. Vasomotor function was assessed in isolated coronary ring chambers. Hearts were randomized into 3 groups: group I (n = 5), cardioplegic arrest, 12-hour storage at 4 degrees C with modified Belzer solution, and 2-hour sanguineous reperfusion in the working state; group II (n = 6), 12-hour continuous perfusion in the beating working state, 30 minutes of arrest (to simulate re-implantation time), and 2 hours of reperfusion, as above; group III (n = 7), coronary ring control hearts. RESULTS: At 2 hours of reperfusion, left ventricular developed pressure in group II was higher than in group I (mean +/- standard deviation: 90 +/- 6 mm Hg, 53 +/- 15 mm Hg, P = .005). Significantly less myocardial edema was observed in group II than in group I (73% +/- 4%, 80% +/- 1% water content, P = .01). Significantly less myocardial acidosis was noted in group II than in group I during preservation (pH 7.3 +/- 0.01, 6.1 +/- 0.03, P < .001) and reperfusion (pH 7.3 +/- 0.008, 6.8 +/- 0.05, P < .001). Coronary endothelial vasomotor function was better preserved in group II than in group I as evidenced by dose-response relaxation of coronary rings to 10(-8) mol/L bradykinin (37%, 55% delta baseline, P = .01). CONCLUSION: This new method extends the current preservation limit and avoids time-dependent ischemic injury, thereby allowing for distant procurement of donor organs. |
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Authors:
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W H Hassanein; L Zellos; T A Tyrrell; N A Healey; M D Crittenden; V Birjiniuk; S F Khuri |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of thoracic and cardiovascular surgery Volume: 116 ISSN: 0022-5223 ISO Abbreviation: J. Thorac. Cardiovasc. Surg. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-11-13 Completed Date: 1998-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376343 Medline TA: J Thorac Cardiovasc Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 821-30 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, Brockton/West Roxbury Veterans Administration Medical Center, Harvard Medical School, Boston, Mass 02132, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Coronary Circulation / physiology Energy Metabolism / physiology Heart Arrest, Induced Heart Transplantation / physiology* Male Myocardial Contraction / physiology* Myocardial Reperfusion Injury / physiopathology Organ Preservation* / instrumentation Perfusion Tissue Survival / physiology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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