Document Detail


Continuous intracellular recording from mammalian neurons exposed to hyperbaric helium, oxygen, or air.
MedLine Citation:
PMID:  10926669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a hyperbaric chamber for intracellular recording in rat brain stem slices during continuous compression and decompression of the tissue bath with the inert gas helium. Air, rather than helium, was also used as the compression medium in some cases to increase tissue nitrogen levels. An important feature is the chamber door, which opens or closes rapidly at 1 atmosphere absolute (ATA) for increased accessibility of the microelectrode. The door also closes and seals smoothly without disrupting the intracellular recording. Hyperbaric oxygen was administered during helium compression using a separate pressure cylinder filled with perfusate equilibrated with 2. 3-3.3 ATA oxygen. Measurements of tissue/bath PO(2) and pH confirmed that the effects of compression using helium or air could be differentiated from those due to increased PO(2). One hundred and thirteen neurons were studied during 375 compression cycles ranging from 1 to 20 ATA (mode 3.0 ATA). We conclude that it is technically feasible to record intracellularly from the same mammalian neuron while changing ambient pressure over a physiologically important range. These techniques will be useful for studying how various hyperbaric environments affect neurophysiological mechanisms.
Authors:
J B Dean; D K Mulkey
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  89     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-15     Completed Date:  2000-09-15     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  807-22     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, College of Science and Mathematics, Wright State University School of Medicine, Dayton, Ohio 45435, USA. jay.dean@wright.edu
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MeSH Terms
Descriptor/Qualifier:
Air Pressure*
Animals
Cell Differentiation / physiology
Cerebrospinal Fluid / physiology
Electrophysiology
Female
Helium*
Hydrogen-Ion Concentration
Hyperbaric Oxygenation*
Male
Membrane Potentials / physiology
Neurons / physiology*
Oxygen / blood
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
NSS 2S07-RR-05794-16/RR/NCRR NIH HHS; R01 HL-56683/HL/NHLBI NIH HHS; R29 HL-46308/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
7440-59-7/Helium; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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