| Continuous intracellular recording from mammalian neurons exposed to hyperbaric helium, oxygen, or air. | |
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MedLine Citation:
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PMID: 10926669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a hyperbaric chamber for intracellular recording in rat brain stem slices during continuous compression and decompression of the tissue bath with the inert gas helium. Air, rather than helium, was also used as the compression medium in some cases to increase tissue nitrogen levels. An important feature is the chamber door, which opens or closes rapidly at 1 atmosphere absolute (ATA) for increased accessibility of the microelectrode. The door also closes and seals smoothly without disrupting the intracellular recording. Hyperbaric oxygen was administered during helium compression using a separate pressure cylinder filled with perfusate equilibrated with 2. 3-3.3 ATA oxygen. Measurements of tissue/bath PO(2) and pH confirmed that the effects of compression using helium or air could be differentiated from those due to increased PO(2). One hundred and thirteen neurons were studied during 375 compression cycles ranging from 1 to 20 ATA (mode 3.0 ATA). We conclude that it is technically feasible to record intracellularly from the same mammalian neuron while changing ambient pressure over a physiologically important range. These techniques will be useful for studying how various hyperbaric environments affect neurophysiological mechanisms. |
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Authors:
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J B Dean; D K Mulkey |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 89 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-09-15 Completed Date: 2000-09-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 807-22 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, College of Science and Mathematics, Wright State University School of Medicine, Dayton, Ohio 45435, USA. jay.dean@wright.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Air Pressure* Animals Cell Differentiation / physiology Cerebrospinal Fluid / physiology Electrophysiology Female Helium* Hydrogen-Ion Concentration Hyperbaric Oxygenation* Male Membrane Potentials / physiology Neurons / physiology* Oxygen / blood Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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NSS 2S07-RR-05794-16/RR/NCRR NIH HHS; R01 HL-56683/HL/NHLBI NIH HHS; R29 HL-46308/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-59-7/Helium; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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