Document Detail

Continuous growth and phosphatidylcholine synthesis of rat hepatoma cells in choline-deprived chemically defined medium.
MedLine Citation:
PMID:  3930527     Owner:  NLM     Status:  MEDLINE    
A choline non-required cell line was established from a rat hepatoma cell line. The line designated R-Y121B X cho was able to grow in choline-deprived medium without serum and lipid. Choline is necessary for the biosynthesis of phosphatidylcholine, which is a main component of cell membranes. Phosphatidylcholine can be synthesized by the methylation of phosphatidylethanolamine in liver cells. Phospholipid composition and incorporation of radiolabeled serine into phosphatidylserine or phosphatidylethanolamine were quite similar in R-Y121B X cho and its parental cells. However R-Y121B X cho cells had higher phosphatidylcholine synthesis activity from radiolabeled methionine than parental cells. These results indicate that choline requirement of mammalian cells depends on the activity of phosphatidylethanolamine methyltransferase.
K Yamamoto; A Niwa; Y Yasumura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  125     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-11-20     Completed Date:  1985-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  91-7     Citation Subset:  IM    
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MeSH Terms
Cell Division / drug effects
Cell Line
Choline / metabolism*
Chromosomes / analysis
Culture Media
Liver Neoplasms, Experimental / metabolism*
Methyltransferases / metabolism
Microscopy, Phase-Contrast
Models, Biological
Phosphatidylcholines / biosynthesis*
Phosphatidylethanolamine N-Methyltransferase
Phosphatidylethanolamines / metabolism
Phospholipids / analysis
Serine / metabolism
Reg. No./Substance:
0/Culture Media; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 56-45-1/Serine; 62-49-7/Choline; EC 2.1.1.-/Methyltransferases; EC N-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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