| Continuous differential impedance spectroscopy of single cells. | |
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MedLine Citation:
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PMID: 20927185 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10404-009-0534-2) contains supplementary material, which is available to authorized users. |
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Authors:
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Daniele Malleo; J Tanner Nevill; Luke P Lee; Hywel Morgan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2009-12-10 |
Journal Detail:
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Title: Microfluidics and nanofluidics Volume: 9 ISSN: 1613-4982 ISO Abbreviation: - Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-10-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101480228 Medline TA: Microfluid Nanofluidics Country: - |
Other Details:
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Languages: ENG Pagination: 191-198 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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