Document Detail


Continuous dielectrophoretic separation of cell mixtures.
MedLine Citation:
PMID:  95165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Use of stream-centered dielectrophoresis (1-4) produced continuous separations on three cell mixtures (1) Chorella vulgaris with Netrium digitus, (2) Ankistrodesmus falcatus with Staurastrum gracile, and (3) Saccharomyces cerevisiae with Netrium digitus. Maximal separations were obtained for these mixtures of live cells at 100 kHz, 600 kHz, and 2.0 MHz, respectively. The technique was restricted to a frequency range of 0.01-32 MHz, and to suspensions of low conductivity in which microorganisms such as these algae and yeast are tolerant. Extension, however, to cellular organisms requiring higher osmolarity is readily feasible through the use of nonionic solutes such as sucrose, mannose, glycine, etc.
Authors:
H A Pohl; K Kaler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell biophysics     Volume:  1     ISSN:  0163-4992     ISO Abbreviation:  Cell Biophys.     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1980-11-24     Completed Date:  1980-11-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002185     Medline TA:  Cell Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15-28     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algae / cytology
Cell Separation / methods*
Chlorella / cytology
Electricity
Electrophoresis / methods*
Mathematics
Saccharomyces cerevisiae / cytology

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