Document Detail

Continuous and sensitive acid phosphatase assay based on a conjugated polyelectrolyte.
MedLine Citation:
PMID:  22812479     Owner:  NLM     Status:  MEDLINE    
We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydrolysis of the substrate pNPP was monitored by the fluorescence change of PPE4+ and corresponding kinetic parameters were derived to be consistent with the literature reports. The applications of PPE4+/pNPP-based ACP assay in high-throughput screening of ACP inhibitors and detection of prostatic acid phosphotase (PAP) in vitro were demonstrated.
Yonghua Xie; Ying Tan; Renxuan Liu; Rui Zhao; Chunyan Tan; Yuyang Jiang
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Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't     Date:  2012-07-25
Journal Detail:
Title:  ACS applied materials & interfaces     Volume:  4     ISSN:  1944-8252     ISO Abbreviation:  ACS Appl Mater Interfaces     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-22     Completed Date:  2012-11-13     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  101504991     Medline TA:  ACS Appl Mater Interfaces     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3784-7     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / chemistry*
Aniline Compounds / chemistry
Drug Design
Electrolytes / chemistry*
Models, Chemical
Nitrophenols / chemistry*
Organophosphorus Compounds / chemistry*
Phosphoric Monoester Hydrolases / chemistry
Polymers / chemistry
Prostatic Neoplasms / diagnosis
Protein Tyrosine Phosphatases / chemistry
Spectrometry, Fluorescence / methods
Reg. No./Substance:
0/Aniline Compounds; 0/Electrolytes; 0/Nitrophenols; 0/Organophosphorus Compounds; 0/Polymers; 330-13-2/nitrophenylphosphate; 72962-65-3/4-aminophenylphosphate; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC Phosphatase; EC acid phosphatase; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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