| Continuous and sensitive acid phosphatase assay based on a conjugated polyelectrolyte. | |
| | |
MedLine Citation:
|
PMID: 22812479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydrolysis of the substrate pNPP was monitored by the fluorescence change of PPE4+ and corresponding kinetic parameters were derived to be consistent with the literature reports. The applications of PPE4+/pNPP-based ACP assay in high-throughput screening of ACP inhibitors and detection of prostatic acid phosphotase (PAP) in vitro were demonstrated. |
| | |
Authors:
|
Yonghua Xie; Ying Tan; Renxuan Liu; Rui Zhao; Chunyan Tan; Yuyang Jiang |
Related Documents
:
|
19344909 - Model-based design of a pilot-scale simulated moving bed for purification of citric aci... 8436059 - Clinical evaluation of a citric acid inhaler for smoking cessation. 3304289 - Short-term control of the pentose phosphate cycle by insulin could be modulated by the ... 15945869 - Evaluation of a rapid technique for antibody elution. 16668249 - Mechanism of aluminum tolerance in snapbeans : root exudation of citric acid. 22026389 - Occurrence and stability of inorganic and organic arsenic species in wines, rice wines ... 386739 - Rabbit lung microsomes: a rapid method for assaying simultaneously drug effects on thro... 2834319 - Uptake of fatty acids by mycoplasma capricolum. 8074299 - Determination of manganese peroxidase activity with 3-methyl-2-benzothiazolinone hydraz... |
Publication Detail:
|
Type: Letter; Research Support, Non-U.S. Gov't Date: 2012-07-25 |
Journal Detail:
|
Title: ACS applied materials & interfaces Volume: 4 ISSN: 1944-8252 ISO Abbreviation: ACS Appl Mater Interfaces Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-08-22 Completed Date: 2012-11-13 Revised Date: 2013-03-04 |
Medline Journal Info:
|
Nlm Unique ID: 101504991 Medline TA: ACS Appl Mater Interfaces Country: United States |
Other Details:
|
Languages: eng Pagination: 3784-7 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acid Phosphatase
/
chemistry* Aniline Compounds / chemistry Catalysis Drug Design Electrolytes / chemistry* Humans Hydrolysis Kinetics Male Models, Chemical Nitrophenols / chemistry* Organophosphorus Compounds / chemistry* Phosphoric Monoester Hydrolases / chemistry Polymers / chemistry Prostatic Neoplasms / diagnosis Protein Tyrosine Phosphatases / chemistry Spectrometry, Fluorescence / methods |
| Chemical | |
Reg. No./Substance:
|
0/Aniline Compounds; 0/Electrolytes; 0/Nitrophenols; 0/Organophosphorus Compounds; 0/Polymers; 330-13-2/nitrophenylphosphate; 72962-65-3/4-aminophenylphosphate; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.2/Acid Phosphatase; EC 3.1.3.2/prostatic acid phosphatase; EC 3.1.3.48/Protein Tyrosine Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lnk deficiency partially mitigates hematopoietic stem cell aging.
Next Document: Tobacco mosaic virus as a new carrier for tumor associated carbohydrate antigens.