Document Detail


Continuous and sensitive acid phosphatase assay based on a conjugated polyelectrolyte.
MedLine Citation:
PMID:  22812479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a novel continuous and sensitive fluorescence turn-on assay for ACPs, which consists of a cationic conjugated polyelectrolyte (PPE4+) and a commonly used phosphatase substrate p-nitrophenyl phosphate (pNPP). The kinetics of the ACP catalyzed hydrolysis of the substrate pNPP was monitored by the fluorescence change of PPE4+ and corresponding kinetic parameters were derived to be consistent with the literature reports. The applications of PPE4+/pNPP-based ACP assay in high-throughput screening of ACP inhibitors and detection of prostatic acid phosphotase (PAP) in vitro were demonstrated.
Authors:
Yonghua Xie; Ying Tan; Renxuan Liu; Rui Zhao; Chunyan Tan; Yuyang Jiang
Related Documents :
19344909 - Model-based design of a pilot-scale simulated moving bed for purification of citric aci...
8436059 - Clinical evaluation of a citric acid inhaler for smoking cessation.
3304289 - Short-term control of the pentose phosphate cycle by insulin could be modulated by the ...
15945869 - Evaluation of a rapid technique for antibody elution.
16668249 - Mechanism of aluminum tolerance in snapbeans : root exudation of citric acid.
22026389 - Occurrence and stability of inorganic and organic arsenic species in wines, rice wines ...
386739 - Rabbit lung microsomes: a rapid method for assaying simultaneously drug effects on thro...
2834319 - Uptake of fatty acids by mycoplasma capricolum.
8074299 - Determination of manganese peroxidase activity with 3-methyl-2-benzothiazolinone hydraz...
Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't     Date:  2012-07-25
Journal Detail:
Title:  ACS applied materials & interfaces     Volume:  4     ISSN:  1944-8252     ISO Abbreviation:  ACS Appl Mater Interfaces     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-22     Completed Date:  2012-11-13     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  101504991     Medline TA:  ACS Appl Mater Interfaces     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3784-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / chemistry*
Aniline Compounds / chemistry
Catalysis
Drug Design
Electrolytes / chemistry*
Humans
Hydrolysis
Kinetics
Male
Models, Chemical
Nitrophenols / chemistry*
Organophosphorus Compounds / chemistry*
Phosphoric Monoester Hydrolases / chemistry
Polymers / chemistry
Prostatic Neoplasms / diagnosis
Protein Tyrosine Phosphatases / chemistry
Spectrometry, Fluorescence / methods
Chemical
Reg. No./Substance:
0/Aniline Compounds; 0/Electrolytes; 0/Nitrophenols; 0/Organophosphorus Compounds; 0/Polymers; 330-13-2/nitrophenylphosphate; 72962-65-3/4-aminophenylphosphate; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.2/Acid Phosphatase; EC 3.1.3.2/prostatic acid phosphatase; EC 3.1.3.48/Protein Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Lnk deficiency partially mitigates hematopoietic stem cell aging.
Next Document:  Tobacco mosaic virus as a new carrier for tumor associated carbohydrate antigens.