Document Detail


The continuing evolution of the Langendorff and ejecting murine heart: new advances in cardiac phenotyping.
MedLine Citation:
PMID:  22636675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isolated retrograde-perfused Langendorff heart and the isolated ejecting heart have, over many decades, resulted in fundamental discoveries that form the underpinnings of our current understanding of the biology and physiology of the heart. These two experimental methodologies have proven invaluable in studying pharmacological effects on myocardial function, metabolism, and vascular reactivity and in the investigation of clinically relevant disease states such as ischemia-reperfusion injury, diabetes, obesity, and heart failure. With the advent of the genomics era, the isolated mouse heart preparation has gained prominence as an ex vivo research tool for investigators studying the impact of gene modification in the intact heart. This review summarizes the historical development of the isolated heart and provides a practical guide for the establishment of the Langendorff and ejecting heart preparations with a particular emphasis on the murine heart. In addition, current applications and novel methods of recording cardiovascular parameters in the isolated heart preparation will be discussed. With continued advances in methodological recordings, the isolated mouse heart preparation will remain physiologically relevant for the foreseeable future, serving as an integral bridge between in vitro assays and in vivo approaches.
Authors:
Ronglih Liao; Bruno K Podesser; Chee Chew Lim
Related Documents :
8287455 - Multiple spontaneous coronary artery dissections in a middle aged woman: support for an...
15700195 - Recent insights into the mechanisms, predisposing factors, and racial differences of co...
220865 - Altered adrenergic activity in coronary arterial spasm: insight into mechanism based on...
9538315 - Myocardial infarction in young people with normal coronary arteries.
23173585 - Microvascular function/dysfunction downstream a coronary stenosis.
3963215 - Adenosine deaminase attenuates canine coronary vasodilation during systemic hypoxia.
Publication Detail:
Type:  Historical Article; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-05-25
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  303     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2012-10-09     Revised Date:  2014-05-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H156-67     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Anura
Cardiac Electrophysiology / history
Heart / physiology*
History, 19th Century
History, 20th Century
History, 21st Century
Humans
Mice
Mice, Mutant Strains
Myocardial Contraction / physiology
Perfusion / methods
Stroke Volume*
Tissue and Organ Harvesting / history
Grant Support
ID/Acronym/Agency:
HL-086967/HL/NHLBI NIH HHS; HL-088533/HL/NHLBI NIH HHS; HL-093148/HL/NHLBI NIH HHS; HL-095813/HL/NHLBI NIH HHS; HL-099073/HL/NHLBI NIH HHS; R01 HL095813/HL/NHLBI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Angiotensin II enhances AT1-Nox1 binding and stimulates arterial smooth muscle cell migration and pr...
Next Document:  Protein kinase D increases maximal Ca2+-activated tension of cardiomyocyte contraction by phosphoryl...