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Continued initiation of DNA synthesis in arginine-deprived Chinese hamster ovary cells.
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MedLine Citation:
PMID:  853059     Owner:  NLM     Status:  MEDLINE    
When exponentially growing CHO cells were deprived of arginine (Arg), cell multiplication ceased after 12 h, but initiation of DNA synthesis continued: after 48 h of starvation with continuous [3H]thymidine exposure, 85% of the population had incorporated label, as detected autoradiographically. Consideration of the distribution of exponential cells in the various cell cycle phases leads to a calculation that most cells in G1 at the time that Arg was removed, as well as those in S, engaged in some DNA synthesis during starvation. In contrast, isoleucine (Ile)-starved cells did not initiate DNA synthesis, as has been reported by others. Experiments with cells synchronized by mitotic selection confirmed this difference in Arg- and Ile- deprived behavior, but also showed that cells which underwent the mitosis leads to G1 transition during Arg starvation remained arrested in G1 (G0?). The results suggest that Arg-deprived cells continue to maintain some proliferative function(s) while Ile-deprived cells do not.
A S Weissfeld; H Rouse
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  73     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1977 Apr 
Date Detail:
Created Date:  1977-06-11     Completed Date:  1977-06-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  200-5     Citation Subset:  IM    
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MeSH Terms
Arginine / metabolism*
Cell Division
Cell Line
Cell Survival
DNA / biosynthesis*
Isoleucine / metabolism
Reg. No./Substance:
73-32-5/Isoleucine; 74-79-3/Arginine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 4 Year: 1977
Volume: 73 Issue: 1
First Page: 200 Last Page: 205
ID: 2109890
Publisher Id: 77165385
PubMed Id: 853059

Continued initiation of DNA synthesis in arginine-deprived Chinese hamster ovary cells

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