| Continentalic acid from Aralia continentalis induces growth inhibition and apoptosis in HepG2 cells. | |
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MedLine Citation:
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PMID: 18806961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we investigated the effects of continentalic acid (CA, (-)-pimara-8(14), 15-diene-19-oic acid), a diterpenic acid, isolated from Aralia continentalis, on the proliferation and apoptosis induction of HepG2 cells. In 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, the inhibitory effect became gradually stronger with the passage of time, 24, 48 and 72 h after treatment with CA, and the most significant effect was observed at 72 h. CA treatment for 72 h induced DNA fragmentation in a dose-dependent manner. Furthermore, flow cytometric analysis of HepG2 cells exposed to CA showed that apoptotic cells increased in a dose-dependent manner. The induction of apoptosis in HepG2 cells by CA was mediated through the activation of caspase-3, Bak, and Bax, and then through the cleavage of peroxisome proliferator-activated receptor (PARP) and the down-regulation of Bcl-2. These results demonstrate that CA efficiently induces apoptosis and is a good candidate for further evaluation as an effective chemotherapeutic agent. |
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Authors:
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Tae Oh Kwon; Seung-Il Jeong; Ji Wung Kwon; Youn Chul Kim; Seon Il Jang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-20 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 31 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-22 Completed Date: 2008-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 1172-8 Citation Subset: IM |
Affiliation:
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Medicinal Resources Research Institute, Wonkwang University, Iksan, 570-749, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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isolation & purification,
pharmacology* Apoptosis / drug effects* Aralia / chemistry* Blotting, Western Caspase 3 / metabolism Cell Line, Tumor DNA Fragmentation / drug effects Diterpenes / isolation & purification, pharmacology* Flow Cytometry Fluorescent Dyes Humans Indoles L-Lactate Dehydrogenase / metabolism Protein Synthesis Inhibitors / pharmacology Tetrazolium Salts Thiazoles |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Diterpenes; 0/Fluorescent Dyes; 0/Indoles; 0/Protein Synthesis Inhibitors; 0/Tetrazolium Salts; 0/Thiazoles; 0/continentalic acid; 298-93-1/thiazolyl blue; 47165-04-8/DAPI; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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