Document Detail

Context-specific regulation of LINE-1.
MedLine Citation:
PMID:  17903170     Owner:  NLM     Status:  MEDLINE    
The present study was conducted to evaluate the contextual specificity of long interspersed nuclear element-1 (LINE-1 or L1) activation by cellular stress and the role of the aryl hydrocarbon receptor (AHR) transcription factor and oxidative stress in the gene activation response. Activation of the AHR by the genotoxic carcinogen benzo(a)pyrene (BaP) increased L1 expression in human cervical carcinoma (HeLa) cells, human microvascular endothelial cells (HMEC), mouse vascular smooth muscle cells (mVSMC) and mouse embryonic kidney cells (mK4). In contrast, challenge with a different AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or UV irradiation (10-20 J/m(2)), induced L1 only in HeLa cells. Transactivation of the mouse L1Md-A5 promoter was observed in all cell types challenged with BaP, while TCDD was without effect, and UV only activated L1 in HeLa cells. Genetic and pharmacological experiments implicated the AHR and oxidative stress as contextual determinants of L1 inducibility by cellular stress.
Ivo Teneng; Vilius Stribinskis; Kenneth S Ramos
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Genes to cells : devoted to molecular & cellular mechanisms     Volume:  12     ISSN:  1356-9597     ISO Abbreviation:  Genes Cells     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2007-11-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9607379     Medline TA:  Genes Cells     Country:  England    
Other Details:
Languages:  eng     Pagination:  1101-10     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, and Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Louisville, KY 40292, USA.
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MeSH Terms
Benzo(a)pyrene / chemistry
Cell Line
Endothelial Cells / cytology
Gene Expression Regulation*
Hela Cells
Long Interspersed Nucleotide Elements / genetics,  physiology*
Models, Biological
Myocytes, Smooth Muscle / cytology
Oxidative Stress
Promoter Regions, Genetic
Tetrachlorodibenzodioxin / pharmacology
Transcriptional Activation
Ultraviolet Rays
Grant Support
Reg. No./Substance:
0/Ligands; 1746-01-6/Tetrachlorodibenzodioxin; 50-32-8/Benzo(a)pyrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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